水产品中地西泮快速检测产品技术评价

Technical evaluation of the rapid detection of diazepam in the aquatic products

中文关键词:  水产品，地西泮，快速检测产品，技术评价

英文关键词:Center for Quality and Safety of Agricultural Products of Beijing

中文摘要:

      目的 对北京市场认知度较高的5个厂家地西泮快速检测产品进行评价。方法 本研究以鲤鱼、草鱼、鲫鱼为基质从灵敏度、钩状效应(HOOK效应)、特异性、准确性、操作简便性等方面进行技术评价, 评价结果参照中国水产科学研究院关于印发《2023年度水产品中药物残留快速检测产品现场验证的通知》。结果 I~V公司检出限灵敏度分别为: 92.2%、88.9%、100.0%、65.6%、33.3%; II公司HOOK效应假阴性10.0%; 其他4家公司均未出现HOOK效应; III公司对4种常见兽药残留均有交叉反应, 交叉反应率高达60%~90%, II公司对恩诺沙星交叉反应率为10%, V公司对恩诺沙星、土霉素有交叉反应, 交叉反应率分别为10.0%、20.0%; 其他2家公司均未出现交叉反应; 所有样品总体准确率在79.2%~96.9%之间, 总体准确率最高的为I公司, 准确率96.9%, 最低的为V公司, 准确率79.2%; 24样品测试平均耗时在57~82 min之间, 满足快速检测技术的要求。I公司水产品中地西泮快速检测产品评价合格; II~V公司评价均不合格, 目前北京市销售的水产品中地西泮快速检测产品性能仍与国家法定确证方法有一定差距, 需继续改进。结论 本研究以期为基层监管工作人员选购及应用提供参考, 推进快速检测技术在水产品质量安全监管中的应用

英文摘要:

      Objective To evaluate the diazepam rapid detection products of 5 manufacturers with high recognition in Beijing. Methods In this study, common carp, grass carp and crucian carp were used as the substrate for technical evaluation from the aspects of sensitivity, HOOK effect, specificity, accuracy and ease of operation, etc. The evaluation results were referred to the Notice on the field verification of rapid detection products for drug residues in aquatic products in 2023 issued by the Chinese Academy of Fishery Sciences. Results The limits of detection for sensitivity of company I-V were 92.2%, 88.9%, 100.0%, 65.6% and 33.3%, respectively. Company II HOOK effect false negative 10%; HOOK effect did not appear in the other 4 companies. The cross-reaction rate of company III to the residues of 4 kinds of common veterinary drugs was as high as 60% to 90%, the cross-reaction rate of company II to enrofloxacin was 10%, the cross-reaction rate of company V to enrofloxacin and oxytetracycline were 10.0% and 20.0%. The other 2 companies did not have cross-reaction. The overall accuracy of all samples ranged from 79.2% to 96.9%. Company I had the highest overall accuracy (96.9%) and company V had the lowest accuracy (79.2%). The average test time of 24 samples is between 57 and 82 min, which meet the requirements of rapid detection technology. The rapid detection of diazepam in aquatic products of company I was qualified. The evaluation of company II-V was not qualified. At present, the performance of diazepam rapid detection products in aquatic products sold in Beijing was still a certain gap with the national legal confirmation method, which needed to be further improved. Conclusion This study is expected to provide reference for the purchase and application of grassroots supervision staff, and promote the application of rapid detection technology in the supervision of aquatic product quality and safety.