柱前衍生-高效液相色谱法测定特殊配方食品中的牛磺酸

Determination of taurine in special formula foods by pre column derivative high performance liquid chromatography

中文关键词:  氨基酸配方及蛋白水解配方  牛磺酸  柱前衍生  实心核颗粒技术  高效液相色谱

英文关键词:amino acid formula and protein hydrolyzed formula foods  taurine  pre column derivatization  solid core particle technology  high performance liquid chromatography

中文摘要:

      目的 建立实心核颗粒技术-柱前衍生-高效液相色谱法测定氨基酸配方及蛋白水解配方等特殊配方食品中牛磺酸的分析方法。方法 样品加水溶解提取后沉淀蛋白, 上清液与足量丹磺酰氯衍生反应; 采用实心核颗粒C18色谱柱分离, 流动相为乙腈-乙酸钠溶液, 梯度洗脱, 高效液相色谱荧光检测器测定, 外标法定量。结果 牛磺酸峰型良好, 与杂质基线分离, 在0~40.0 μg/mL范围内线性良好(r2>0.999), 方法检出限和定量限分别为0.04 mg/100 g、0.10 mg/100 g; 在低、中、高3个浓度水平下的平均回收率范围为90.5%~105.4%, 相对标准偏差为0.5%~8.1%。结论 该方法准确度高、重复性和稳定性好, 适用于氨基酸配方及蛋白水解配方等特殊配方食品的检测, 解决了国标方法检测氨基酸配方及深度水解蛋白乳粉等特殊配方食品中牛磺酸方法的不足。

英文摘要:

      Objective To establish a method for the determination of taurine in special formula foods such as amino acid formula and protein hydrolysate using solid-core particle technology-precolumn derivatization-high performance liquid chromatography. Methods After the samples were dissolved in water and the protein was precipitated, the supernatant was taken for derivative reaction with dansyl chloride. Later, samples were separated on a Cortecs C18 column and gradient eluted with acetonitrile-sodium acetate solution as mobile phase, determined by fluorescence detector and quantified by external standard method. Results The results showed that the peak of taurine was good and separated from the impurity baseline, with good linearity in the range of 0~40.0 μg/mL (r2>0.999), and the limit of detection and limit of quantification were 0.04 mg/100 g and 0.10 mg/100 g, respectively. The average recoveries of taurine at low, medium and high spiked levels were 90.5%~105.4%, and the relative standard deviations were 0.5%~8.1%. Conclusion This method has high accuracy, good repeatability and stability, and is suitable for the detection of special formula foods such as amino acid formulas and protein hydrolysis formulas. It solves the shortcomings of the national standard method for detecting taurine in amino acid formulas and deeply hydrolyzed protein milk powder.