水飞蓟复方制剂对急性酒精性肝损伤的保护作用研究

Research on the protective effects of silymarin compound preparation on acute alcoholic liver injury

中文关键词:  水飞蓟  复方制剂  急性酒精性肝损伤

英文关键词:silymarin  compound preparation  acute alcoholic liver injury

中文摘要:

      目的 探究水飞蓟复方制剂对急性酒精性肝损伤小鼠的保护作用。方法 将60只雄性KM小鼠随机分为空白对照组, 模型组, 水飞蓟复方制剂低、中、高剂量组, 共5组, 每组12只。空白对照组和模型组灌胃相同体积的无菌水, 其余各组灌胃对应的水飞蓟复方制剂溶液, 在灌胃的第28 d, 除空白对照组外, 其余组小鼠每隔12 h灌胃50%酒精, 共计3次, 诱导小鼠急性酒精性肝损伤模型。采用苏木精-伊红(hematoxylin eosin, HE)染色观察小鼠肝脏病理学变化; 测定小鼠肝脏中丙二醛(malondialdehyde, MDA)、谷胱甘肽(glutathione, GSH)、甘油三酯(triglyceride, TG)的含量和谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)、总超氧化物歧化酶(total superoxide dismutase, T-SOD)、过氧化氢酶(catalase, CAT)的活性, 测定小鼠血清中谷丙转氨酶(alanine aminotransferase, ALT)和谷草转氨酶(aspartate aminotransferase, AST)的活性以及白细胞介素-1β (interleukin-1β, IL-1β)、白细胞介素-6 (interleukin-6, IL-6)、肿瘤坏死因子-α (tumor necrosis factor-α, TNF-α)的水平。结果 HE染色显示水飞蓟复方制剂组中的肝细胞水肿、气球样变现象得到改善; 与模型组相比, 在水飞蓟复方制剂高剂量组中, GSH含量显著升高(P<0.05), 在其中剂量组中, T-SOD、CAT活性显著升高(P<0.05), MDA含量显著降低(P<0.05); 利用生化分析仪测定水飞蓟复方制剂的转氨酶活性, 发现在其中、高剂量组中的ALT、AST活性显著降低(P<0.01); 酶联免疫吸附(enzyme linked immunosorbent assay, ELISA)法测定各组炎症因子水平, 显示水飞蓟复方制剂低剂量组中的IL-1β水平和中、低剂量组中TNF-α水平显著降低(P<0.01)。结论 水飞蓟复方制剂通过提高肝脏的抗氧化能力、降低血液中转氨酶活性、抑制促炎细胞因子的产生, 从而在急性酒精性肝损伤中发挥一定的保护作用, 为相关保健食品的研发应用提供一定价值的数据支持。

英文摘要:

      Objectives To investigate the protective effects of silymarin compound preparation on acute alcoholic liver injury in mice. Methods The 60 male KM mice were randomly divided into 5 groups of 12 mice each, including the blank group, model group, silymarin compound preparation low-dose group, medium-dose group, high-dose group. The blank group and model group were gavaged with the same volume of sterile water, and the remaining groups were gavaged with the corresponding silymarin compound preparation solution, on the 28th day of gavage, except for the blank group, the rest of the groups of mice were gavaged with 50% alcohol every 12 h for a total of 3 times to induce the acute alcoholic liver injury model in mice. Hematoxylin eosin (HE) staining was used to observe the pathological changes of mouse liver; the content of malondialdehyde (MDA), glutathione (GSH) and triglyceride (TG) and the activities of glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD) and catalase (CAT) in mouse liver were determined, and the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as well as the levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) levels were determined. Results HE staining showed that hepatocyte oedema and balloon-like changes were improved in the group of silymarin compound preparation; compared with the model group, GSH content was significantly higher in the high dose group of silymarin compound preparation (P<0.05), and in its middle dose group, the activities of T-SOD and CAT were significantly higher (P<0.05), and the content of MDA was significantly lower (P<0.05); the levels of transcripts were determined by using a biochemical analyzer to determine the transaminase activity of the silymarin compound preparation, found that in its medium and high dose groups, ALT and AST activity was significantly reduced (P<0.01); The level of inflammatory factors in each group was determined by enzyme linked immunosorbent assay (ELISA) method, showed that the level of IL-1β in the low dose group of the silymarin compound preparation and the level of TNF-α in the medium and low dose group were significantly reduced (P<0.01). Conclusion Silymarin compound preparation can play a certain protective role in acute alcoholic liver injury by improving the antioxidant capacity of the liver, reducing the activity of transaminases in the blood, and inhibiting the production of pro-inflammatory cytokines, thus providing some valuable data support for the research and development application of the relevant health food.