吡虫啉和啶虫脒胶体金免疫层析法试纸条性能评估及应用效果探究

Performance evaluation and application effect study of colloidal gold immunochromatographic test strips for imidacloprid and acetamiprid

中文关键词:  吡虫啉  啶虫脒  胶体金免疫层析法  快速检测

英文关键词:imidacloprid  acetamiprid  colloidal gold immunochromatography  quick detection

中文摘要:

      目的 研究吡虫啉和啶虫脒胶体金免疫层析法试纸条在不同基质中残留量检测的应用效果。方法 实验考察了市场上主要流通的5种不同品牌的胶体金免疫层析法试纸条在蔬菜、水果和茶鲜叶基质中的检出限、灵敏度、交叉反应率、特异性、假阳性和假阴性率。结果 各品牌胶体金试纸条在3种不同类型基质(白菜、苹果和茶鲜叶)中, 检出限浓度水平有假阴性出现, 其余浓度水平灵敏度较好; 特异性和交叉反应性, 结果均为阴性; 假阳性率和假阴性率考察中, 假阳性率为0%, 但有假阴性情况出现。结论 胶体金免疫层析法在检测吡虫啉、啶虫脒农药残留时具有简便快捷的特点, 在蔬菜、水果基质的检测中, 判定结果较为准确可靠; 在茶叶基质中, 由于基质效应的影响, 出现假阴性的机率较高。

英文摘要:

      Objective To study the application effect of imidacloprid and acetamiprid colloidal gold immunochromatographic test strips for residual detection in different matrices. Methods The experiment examined the detection limit, sensitivity, cross reaction rate, specificity, false positive and false negative rates of 5 kinds of different brands of colloidal gold immunochromatographic test strips circulating in the market in vegetable, fruit, and tea fresh leaf matrices. Results In 3 different types of substrates (apples, cabbage, and fresh tea leaves), false negative results were observed at the limit of detection of concentration level for each brand of colloidal gold test strips, while the sensitivity of the other concentration levels was good; both specificity and cross reactivity were negative. In the investigation of false positive rate and false negative rate, the false positive rate was 0%, but there were cases of false negative. Conclusion The colloidal gold immunochromatography method has the characteristics of simplicity and speed in detecting imidacloprid and acetamiprid pesticide residues. In the detection of vegetable and fruit, the judgment results are relatively accurate and reliable, in fresh tea leaves, due to the influence of matrix effect, the probability of false negatives is higher.