三叶青茎部不同溶剂提取物的功能成分、抗氧化及酪氨酸酶抑制活性研究

Functional components, antioxidant activity and tyrosinase inhibitory activity of extracts from different solvent extracts from stems of Tetrastigma hemsleyanum Diels et Gilg

中文关键词:  三叶青茎部  抗氧化活性  酪氨酸酶抑制活性  相关性

英文关键词:Tetrastigma hemsleyanum Diels et Gilg stems  antioxidant activity  tyrosinase inhibitory activity  correlation

中文摘要:

      目的 以三叶青茎部为原料, 探究三叶青不同溶剂提取物的功能成分、抗氧化及酪氨酸酶抑制活性。方法 采用乙醇超声法提取三叶青, 获得醇提液及残渣。醇提液依次用石油醚、乙酸乙酯、正丁醇对其进行分步萃取, 残渣则采用蒸馏水多次提取, 最终得到4种提取物。分别测定4种提取物总酚、总黄酮、总皂苷、总多糖、抗氧化及酪氨酸酶抑制活性, 并进行相关性分析。结果 4种提取物中乙酸乙酯萃取物总酚、总黄酮、总皂苷含量最高, 分别为40.22%±1.01%、38.56%±0.64%、6.15%±0.21%, 正丁醇萃取物总多糖含量最高, 为9.29%±0.25%。各萃取物均具有抗氧化和酪氨酸酶抑制活性, 其中乙酸乙酯萃取物清除1,1-二苯基-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基、超氧阴离子、2-苯基-4,4,5,5-四甲基咪唑啉-3-氧代-1-氧(2-phenyl-4,4,5,5-tetramethylimidazoline-3-oxide-1-oxyl, PTIO)自由基、羟自由基能力和铁离子还原能力(ferric reducing antioxidant power, FRAP)最强, 其半抑制浓度(half maximal inhibitory concentration, IC50)分别为0.016、0.014、0.355、0.265、0.352 mg/mL。同时, 在酪氨酸酶抑制活性上, 乙酸乙酯萃取物明显强于各萃取物。相关性分析表明, 三叶青提取物中总酚、总黄酮、总皂苷物质与抗氧化和酪氨酸酶抑制活性呈极显著正相关性(P<0.01)。结论 三叶青茎部乙酸乙酯萃取物具有较强的抗氧化性及抑制酪氨酸酶活性, 主要作用成分为总酚、总黄酮和总皂苷。本研究为进一步开发利用三叶青茎部资源提供相关技术支持。

英文摘要:

      Objective To investigate the functional components, antioxidant and tyrosinase inhibitory activity of different solvent extracts from Tetrastigma hemsleyanum Diels et Gilg by using the stem of Tetrastigma hemsleyanum Diels et Gilg as the raw material. Methods The ultrasonification process and the absolute ethyl alcohol were used to deal with the stem of Tetrastigma hemsleyanum Diels et Gilg, obtaining the residues and alcohol extracts. The distilled water multiply extracted the residues, while the alcohol extract were extracted sequentially using a three-solvent combination order: Petroleum ether, ethyl acetate, and n-butanol. Finally, 4 kinds of extracts were obtained. The total content of flavonoids, polyphenols, saponin, or other functional components, their corresponding antioxidant activities, and tyrosinase inhibitory activities of 4 kinds of extracts were measured, respectively, then the correlation analysis between in them were conducted. Results The results demonstrated that the extract obtained from the ethyl acetate contained the highest amount of flavonoids, polyphenols, and saponins, and the proportions reached 40.22%±1.01%, 38.56%±0.64%, and 6.15%±0.21%, respectively. The extract obtained from the n-butanol contained the highest amount of polysaccharides, and the proportion reached 9.29%±0.25%. Each extract owned antioxidant activity and tyrosinase inhibitory activity, but the ethyl acetate extract had the most robust ability to 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide anion, 2-phenyl-4,4,5,5-tetramethylimidazoline-3-oxide-1-oxyl (PTIO) reactive oxygen radical, hydroxyl radical, and ferric reducing antioxidant power (FRAP) among all the extracts, and the half inhibitory concentration (IC50) were 0.016, 0.014, 0.355, 0.265 and 0.352 mg/mL, respectively. Moreover, the ethyl acetate extract was superior to other extracts in tyrosinase inhibition activity. The correlation analysis showed that there was a highly significant positive correlation between the total phenols, total flavonoids, and total saponins in the extract of Tetrastigma hemsleyanum Diels et Gilg and its antioxidant and tyrosinase inhibitory activities (P<0.01). Conclusion The ethyl acetate extract from the stem of Tetrastigma hemsleyanum Diels et Gilg has strong antioxidant activity and tyrosinase inhibitory activity. The main active components are total phenols, total flavonoids, and total saponins. This study provides relevant technical supports for the further development and utilization of the stem resources of Tetrastigma hemsleyanum Diels et Gilg.