贝类毒素大田软海绵酸的免疫分析检测技术研究进展

Recent progress in immunoassay detection technology of shellfish-toxin okadaic acid

中文关键词:  生物毒素  腹泻性贝类毒素  大田软海绵酸  免疫分析检测

英文关键词:biotoxin  diarrheal shellfish poison  okadaic acid  immunoassay detection

中文摘要:

      大田软海绵酸(okadaic acid, OA)是一种广泛存在于贝类等生物中的海洋生物毒素, 可引起人或动物的急性中毒, 对食品安全和海产养殖具有严重危害。因此建立快速、可靠、灵敏的OA检测技术具有重要意义。免疫分析检测技术基于抗原抗体的结合, 特异性强、灵敏度高、应用范围广, 是当前检测贝类毒素OA的主要手段。本文综述了近年来针对贝类毒素OA的免疫分析检测技术, 其中包括酶联免疫吸附检测、免疫层析检测、时间分辨荧光免疫检测和基于免疫传感器的检测技术等。本文着重阐述了不同免疫分析技术的原理及其在OA检测中的实际应用, 同时探讨了免疫分析技术在贝类毒素OA检测方面的挑战和发展趋势, 以期为开发性能更加优异的OA免疫检测技术提供研究思路。

英文摘要:

      Okadaic acid (OA) is a marine biotoxin widely found in shellfish, which can cause acute poisoning in humans or animals, and has serious harm to food safety and marine aquaculture. Therefore, it is of great significance to establish a fast, reliable, and sensitive OA detection technology. Immunoassay detection technology is based on the combination of antigen and antibody, with strong specificity, high sensitivity, and wide application range, which is currently the main method for shellfish-toxin OA detection. This work reviewed the immunoassay techniques for shellfish-toxin OA in recent years, including enzyme-linked immunosorbent assay, immunochromatographic assay, time resolved fluoroimmunoassay, and immunosensor based detection techniques. This paper focused on the principles of different immunoassay techniques and their practical applications in OA detection, and discussed the development trends and challenges of immunoassay techniques in the detection of shellfish-toxin OA, in order to provide more new ideas to the authors for developing OA immunoassay techniques with better performance.