流式细胞术在双壳贝类免疫研究中的应用

Application of flow cytometry in researches on bivalves immune

中文关键词:  流式细胞术  贝类  免疫  血细胞

英文关键词:flow cytometry  shellfish  immune  hemocytes

中文摘要:

      双壳贝类作为重要的水产品经济物种和生态指示物种在全球范围内得到广泛养殖, 由于其具有重要的经济价值及在生态毒理学中的环境指示作用, 其免疫能力逐渐受到越来越多的关注。血细胞作为双壳贝类先天免疫的主要参与者, 其细胞活力、吞噬能力、粘附能力、总血细胞数量及过氧化物酶活性等的检测成为探索双壳贝类免疫研究的重要内容。其中, 流式细胞术(flow cytometry, FCM)具有分析速度快、灵敏度高、检测量大、客观性强等技术特点, 是一种较为理想的细胞学研究工具。本文结合国内外近年来关于FCM在双壳贝类免疫研究中的应用, 介绍了FCM的发展历史、双壳贝类免疫学的研究进展及FCM在双壳贝类免疫研究的应用, 总结了FCM在样品处理和检测中亟待解决的问题, 以期FCM在未来贝类免疫学研究中不断完善和发展, 并在贝类食品质量安全评估中发挥重要作用。

英文摘要:

      Bivalves are widely cultured worldwide as an important economic species of aquatic products and ecological indicator species, and their immunity is gradually receiving more and more attention due to their economic importance and their environmental indicator role in ecotoxicology. As a major player in the innate immunity of bivalve shellfish, the detection of cell viability, phagocytosis, adhesion capacity, total blood cell count and peroxidase activity has become an important part of the research to explore the immunity of bivalve shellfish. Among them, flow cytometry (FCM) is a more ideal tool for cytological research because of its technical characteristics such as fast analysis, high sensitivity, large detection volume and objectivity. This paper introduced the development history of FCM, the research progress of bivalve shellfish immunology and the application of FCM in bivalve shellfish immunology, and summarized the problems to be solved in sample processing and detection, with a view to improving and developing FCM in future shellfish immunology research and playing an important role in shellfish food quality and safety assessment. The results showed that FCM can play an important role in shellfish quality and safety assessment.