表面增强拉曼光谱技术在果蔬真菌毒素残留检测中的研究进展

Research progress on mycotoxin residues in fruits and vegetables by surface-enhanced Raman spectroscopy

中文关键词:  表面增强拉曼光谱技术  真菌毒素  果蔬  快速检测

英文关键词:surface-enhancement Raman spectroscopy  mycotoxin  fruits and vegetables  rapid detection

中文摘要:

      果蔬在采摘后的加工、贮藏、运输等环节中易受到环境中微生物的污染, 造成果蔬腐败变质, 其中部分产毒真菌在增殖过程中会产生大量真菌毒素, 给消费者身心健康造成了严重威胁, 因此建立快速、准确的真菌毒素检测方法对保证果蔬品质安全至关重要。近年来, 表面增强拉曼光谱技术(surface-enhancement Raman spectroscopy, SERS)因具有高灵敏性、快速性、无破坏性、不受水分干扰等优点备受关注。本文阐述了SERS概况和增强机制, 对SERS检测果蔬真菌毒素领域常用的金属溶胶基底和固体复合基底进行了简单介绍, 以检测方法、基底制备技术、检测结果和遗留问题为切入点强调了SERS对不同果蔬食品真菌毒素痕量检测的应用, 并介绍了SERS在真菌毒素检测领域的应用前景和局限性, 对现有问题提出了解决思路, 旨在为今后SERS应用于真菌毒素检测领域提供帮助。

英文摘要:

      Fruits and vegetables are easy to be polluted by microorganisms in the environment during processing, storage, transportation and other links after picking, resulting in the deterioration of fruits and vegetables. Some of the toxin producing fungi will produce a lot of mycotoxins during the proliferation process, posing a serious threat to the physical and mental health of consumers. Therefore, it is crucial to establish a rapid and accurate mycotoxin detection method to ensure the quality and safety of fruits and vegetables. In recent years, surface-enhanced Raman spectroscopy (SERS) has attracted much attention due to its high sensitivity, rapidity, non destructiveness, and non water interference. This paper described the general situation and enhancement mechanism of SERS, briefly introduced the metal sol substrate and solid composite substrate commonly used in the field of SERS detection of fruit and vegetable mycotoxins, emphasized the application of SERS to trace detection of mycotoxins in different fruit and vegetable foods, and introduced the application prospects and limitations of SERS in the field of mycotoxins detection, starting with detection methods, substrate preparation technology, detection results and remaining problems. This paper proposed the solutions to the existing problems, aiming to provide help for the application of SERS in the field of mycotoxin detection in the future.