一种具有聚集诱导发光性质的二氧化硫荧光探针的构建及应用研究

Construction and application of a sulfur dioxide fluorescent probe with aggregation induced emission properties

中文关键词:  荧光探针  聚集诱导发光  四苯乙烯  二氧化硫

英文关键词:fluorescent probe  aggregation induced emission  tetrastyrene  sulfur dioxide

中文摘要:

      目的 构建一种具有聚集诱导发光(aggregation induced emission, AIE)性质的二氧化硫(sulfur dioxide, SO2)的检测方法。方法 基于荧光探针的设计方法, 在常见具有AIE性质荧光团四苯乙烯中引入苯甲醛用于特异性识别二氧化硫, 通过有机合成的方法得到该探针(SO2P1), 采用核磁氢谱(1hydrogen-nuclear magnetic resonance, 1H NMR)、核磁碳谱(13carbon-nuclear magnetic resonance, 13C NMR)和高分辨质谱法(high resolution mass spectrometry, HRMS)确证其化学结构, 运用荧光光谱仪考察构建的具有AIE性质的荧光探针SO2P1对二氧化硫的检测效果。结果 探针SO2P1是一例具有AIE特性的荧光探针, 对二氧化硫具有专一的荧光选择性并能够克服常见分析物的干扰。探针SO2P1对二氧化硫的识别具有较强的灵敏性, 能够在10 min内完成, 最低检出限为6 μmol/L。结论 探针SO2P1对二氧化硫具有高效专一的识别特性, 并成功地将探针应用于白葡萄酒和啤酒中二氧化硫的直接检测和加标回收, 取得了较为满意的结果, 表明该探针对二氧化硫的识别具有较强的实用价值。

英文摘要:

      Objective To develop a method for the detection of sulfur dioxide (SO2) with aggregation induced emission (AIE). Methods Based on the design method of the fluorescent probe, benzaldehyde was introduced into tetrastyrene, a common fluorescent group with AIE properties, for specific recognition of sulfur dioxide. The probe (SO2P1) was obtained by organic synthesis. Its chemical structure was confirmed by hydrogen-nuclear magnetic resonance (1H NMR), 13carbon-nuclear magnetic resonance (13C NMR) and high resolution mass spectrometry (HRMS). The detection effect of sulfur dioxide with AIE fluorescent probe SO2P1 was investigated by fluorescence spectrometer. Results Probe SO2P1 was a fluorescent probe with AIE characteristics and it had specific fluorescence selectivity for sulfur dioxide and could overcome the interference of common analytes. Probe SO2P1 had strong sensitivity to identify sulfur dioxide and could be completed within 10 minutes with a minimum the limit of detection was 6 μmol/L. Conclusion The probe SO2P1 has high-efficiency and specific identification characteristics for sulfur dioxide, and has been successfully applied to the direct detection and recovery of sulfur dioxide in white wine and beer, indicating that the probe has strong practical value for the detection of sulfur dioxide.