库尔勒香梨酸性多糖PSAP-1的分离纯化及其糖醛酸含量测定

Separation and purification of acidic polysaccharide PSAP-1 from Pyrus sinkiangensis Yu. and its uronic acid content determination

中文关键词:  库尔勒香梨  酸性多糖  分离纯化  红外图谱  糖醛酸

英文关键词:Pyrus sinkiangensis Yu.  acidic polysaccharide  separation and purification  infrared spectrum  uronic acid

中文摘要:

      目的 分离纯化库尔勒香梨酸性多糖(Pyrus sinkiangensis Yu. acidic polysaccharide, PSAP-1), 检测红外吸收并对其所含糖醛酸进行含量测定。方法 经DEAE-650M、HW-55F及Sephacryl S-300色谱柱进行分离纯化PSAP-1, 通过蒽酮-硫酸法测定多糖含量, 红外光谱法确定其化学键或官能团信息, 采用间羟基联苯法测定PSAP-1中糖醛酸的含量。结果 葡萄糖对照品和PSAP-1的吸光度在0.04~0.12 mg/mL质量浓度范围内线性关系良好, PSAP-1含量为61.51%, 精密度、稳定性、重复性和加样回收率的相对标准偏差(relative standard deviations, RSDs)分别为0.2%、1.3%、1.7%、1.4%, 均小于2.0%; 红外图谱显示, PSAP-1中有糖醛酸的特征吸收峰。确定D-半乳糖醛酸和PSAP-1的吸光度在0.035~0.055 mg/mL质量浓度范围内线性关系良好, 糖醛酸的含量为62.78%, 精密度、稳定性、重复性和加样回收率的RSD分别为0.14%、0.31%、1.00%、1.10%, 均小2.0%。结论 本研究从新疆库尔勒香梨中得到多糖含量高、富含糖醛酸的酸性均一多糖组分PSAP-1, 所确定的PSAP-1多糖含量和糖醛酸含量测定方法准确可靠, 为库尔勒香梨的深层研究提供了基础依据。

英文摘要:

      Objective To separate and purify Pyrus sinkiangensis Yu. acidic polysaccharide (PSAP-1) from Pyrus sinkiangensis Yu., and detect infrared absorption and determine the content of uronic acid in it. Methods PSAP-1 was separated and purified by DEAE-650M, HW-55F and Sephacryl S-300 chromatographic columns. The content was determined by anthrone-sulfuric acid method, and the chemical bond or functional group information was determined by infrared spectroscopy, and the content of uronic acid in PSAP-1 was determined by m-hydroxybiphenyl method. Results The absorbances of glucose reference substance and PSAP-1 were in good linear relationships within the mass concentration range of 0.04?0.12 mg/mL. The content of PSAP-1 was 61.51%, and the relative standard deviations (RSDs) of precision, stability, repeatability and sample recoveries were 0.2%, 1.3%, 1.7% and 1.4%, respectively, which were all less than 2.0%. Infrared spectrum showed that there was a characteristic absorption peak of uronic acid in PSAP-1. The absorbances of D-galactouronic acid and PSAP-1 were in good linear relationships within the mass concentration range of 0.035?0.055 mg/mL. The content of uronic acid was 62.78%, and the RSDs of precision, stability, repeatability and sample recoveries were 0.14%, 0.31%, 1.00%, 1.10%, which were all less than 2.0%. Conclusion In this study, PSAP-1, an acidic homogeneous polysaccharide component with high polysaccharide content and rich uronic acid, is obtained from Pyrus sinkiangensis Yu. in Xinjiang. The methods used for the determination of PSAP-1 polysaccharide content and its uronic acid content are accurate and reliable, providing a basic basis for the deep research of Pyrus sinkiangensis Yu..