葱、姜、萝卜和豇豆中戊唑醇残留检测及膳食风险评估

Residue detection and dietary risk assessment of tebuconazole residues in scallion, ginger, radish and cowpea

中文关键词:  戊唑醇  残留量检测  膳食摄入风险评估

英文关键词:tebuconazole  residue detection  risk assessment for dietary residue intake

中文摘要:

      目的 研究葱、姜、萝卜、萝卜叶和豇豆中戊唑醇残留检测方法, 分析相同施药措施下4种蔬菜中残留量差异, 并对最终残留量进行长期膳食摄入和急性膳食摄入风险评估。方法 采用QuEChERS前处理方法进行样品提取, 高效液相色谱-串联质谱法检测; 运用膳食摄入风险评估模型进行急性膳食摄入和长期膳食摄入风险评估。结果 当添加水平是0.01、0.10和1.00 mg/kg时, 葱、姜、萝卜、萝卜叶和豇豆的平均回收率分别为100%~117%、101%~112%、94%~99%、102%~111%和92%~95%, 相对标准偏差分别为2.0%~3.8%、1.8%~7.6%、2.3%~4.3%、1.4%~7.7%和5.7%~6.9%。2个采收间隔期葱、姜、萝卜、萝卜叶和豇豆中戊唑醇残留量分别为<0.01~0.014、<0.01~0.042、<0.01~0.023、0.057~4.13和<0.01~0.079 mg/kg。戊唑醇残留的普通人群国家估计每日摄入量是0.455 mg, 占日允许摄入量的24.1%左右。葱、姜、萝卜、萝卜叶和豇豆中戊唑醇的国家估计短期摄入量分别为0.0337、0.000432、0.000562、0.0169、0.000532 mg/(kg·bw), 占急性参考剂量(acute reference dose, ARfD)百分比分别为0.112%、1.44%、1.87%、56.3%和1.77%。结论 按该试验的施药方式进行施药时, 最终残留量差异与作物种类相关, 试验蔬菜中戊唑醇的残留量对一般人群健康风险在可接受的范围内。

英文摘要:

      Objective To study the detection methods of tebuconazole residues in scallion, ginger, radish, radish leaves and cowpea, analyze the tebuconazole residue differences between 4 kinds of vegetables under the same application measures, and assess risk assessment of long-term dietary intake and acute dietary intake for the final residues. Methods The samples were extracted by QuEChERS pretreatment method, and detected by high performance liquid chromatography-tandem mass spectrometry, acute dietary intake and long-term dietary intake risk were assessed using dietary intake risk assessment model. Results The average recoveries of tebuconazole in scallion, ginger, radish, radish leaves and cowpea at 3 different addition levels of 0.01, 0.10, and 1.00 mg/kg were 100%?117%, 101%?112%, 94%?99%, 102%?111% and 92%-95%, respectively, with relative standard deviations of 2.0%?3.8%, 1.8%?7.6%, 2.3%?4.3%, 1.4%?7.7% and 5.7%?6.9%, respectively. The residue levels in scallion, ginger, radish, radish leaves, and cowpea were <0.01?0.014, <0.01?0.042, <0.01?0.023, 0.057?4.13 and <0.01?0.079 mg/kg, respectively. The national estimated daily intake of residue of tebuconazole was 0.455 mg, representing about 24.1% of the allowable daily intake. The national estimated acute dietary intake of tebuconazole in scallion, ginger, radish, radish leaves, and cowpea were 0.0337, 0.000432, 0.000562, 0.0169 and 0.000532 mg/(kg·bw), and the acute reference dose (ARfD) was calculated according to formula and found to be 0.112%, 1.44%, 1.87%, 56.3% and 1.77%, respectively. Conclusion According to the application method of this test, the final residue difference is related to the crop type, the residues of tebuconazole in the tested vegetables were within acceptable health risks for the general population.