高效液相色谱法同时测定麻花中16种多环芳烃含量

Simultaneous determination of 16 kinds of polycyclic aromatic hydrocarbons in fried dough twists by high performance liquid chromatography

中文关键词:  高效液相色谱法  多环芳烃  麻花

英文关键词:high performance liquid chromatography  polycyclic aromatic hydrocarbons  fried dough twists

中文摘要:

      目的 建立高效液相色谱法测定麻花中16种多环芳烃(polycyclic aromatic hydrocarbons, PAHs)含量的分析方法。方法 样品经正己烷提取, 提取液经弗洛里硅藻土固相萃取小柱串联PAHs专用固相萃取小柱净化, Waters PAH C18柱进行色谱分离, 采用高效液相色谱法测定, 外标法定量。结果 苊烯在5~200 ng/mL范围内呈现良好的线性关系, 相关系数(r)为0.9996, 其他15种多环芳烃在1~200 ng/mL范围内呈现良好的线性关系, 相关系数(r)为0.9985~0.9998。方法的检出限为0.4~2.5 μg/kg。在5、10、25 μg/kg 3个添加水平的平均回收率为63%~95%, 相对标准偏差小于10%。结论 该方法净化效果好, 具有较好的准确度、灵敏度和精密度, 可用于麻花中多环芳烃的测定。

英文摘要:

      Objective To establish a method for the simultaneous determination of 16 kinds of polycyclic aromatic hydrocarbons (PAHs) in fried dough twists by high performance liquid chromatography. Methods The samples were extracted by n-hexane, and purified by Florian diatomite solid phase extraction column and PAHs-specific solid phase extraction column in series. The chromatographic separation was performed on Waters PAH C18 chromatographic column, determined by high performance liquid chromatography, and quantified by external standard method. Results Acenaphthylene showed good linear relationship in the range of 5?200 ng/mL, and the correlation coefficient (r) was 0.9996, other 15 kinds of polycyclic aromatic hydrocarbons showed good linear relationship in the range of 1?200 ng/mL, and the correlation coefficients (r) were 0.9985?0.9998. The limits of detection were 0.4?2.5 μg/kg. The average recoveries at 3 concentration levels of 5, 10, 25 μg/kg were in the range of 63%?95%, and the relative standard deviations were no more than 10%. Conclusion The method has good purification effect, good accuracy, sensitivity and precision, and can be used for the determination of polycyclic aromatic hydrocarbons in fried dough twists.