婴幼儿配方乳粉和发酵乳中沙门氏菌检验方法的比对研究

Comparative study on the detection methods of Salmonella in infant formula milk powder and fermented milk

中文关键词:  婴幼儿配方乳粉  发酵乳  沙门氏菌  标准方法

英文关键词:infant formula milk powder  fermented milk  Salmonella  standard method

中文摘要:

      目的 筛选针对婴幼儿配方乳粉、发酵乳以及环境样品中沙门氏菌污染最灵敏的检测方法。方法 对20种血清型沙门氏菌进行平行检测, 对比美国食品与药品管理局/微生物分析手册(Food and Drug Adminis tration/Bacteriological Analytical Manual, FDA/BAM) Chapter 5: Salmonella、ISO 6579—2017 Microbiology of the food chain-Horizontal method for the detection, enumeration and serotyping of Salmonella和GB 4789.4—2016《食品安全国家标准 食品微生物学检验沙门氏菌检验》沙门氏菌检验方法的检出限、灵敏度, 使用不同基质(婴幼儿配方奶粉、发酵乳和环境样品)人工染菌样品对3个标准方法的样品适用性进行比较与评价。结果 FDA/BAM、ISO 6579—2017、GB 4789.4—2016方法的检出限为10-1~100 CFU/样品。3个标准方法在101 CFU/样品染菌水平灵敏度均为100%; 100 CFU/样品染菌水平, 灵敏度为95%、90%、90%; 10-1 CFU/样品染菌水平, 灵敏度为65%、50%、45%。对于3类人工污染沙门氏菌的样品, FDA/BAM方法的检出率显著高于ISO 6579—2017和GB 4789—2016方法(P＜0.05), ISO 6579—2017和GB 4789.4—2016方法检出率无显著性差异(P>0.05)。结论 3种方法检出限一致, 均可以有效检测食品中沙门氏菌, 污染水平为10-1~100 CFU/样品时, FDA/BAM方法的灵敏度较高。在不同人工污染沙门氏菌食品基质中, FDA/BAM方法的检出率显著高于ISO 6579—2017和GB 4789—2016。

英文摘要:

      Objective To select the most sensitive detection method for Salmonella contamination in infant formula milk powder, fermented milk products and environmental samples. Methods The detection limits and sensitivities of the methods for Salmonella testing from Food and Drug Administration/Bacteriological Analytical Manual (FDA/BAM) Chapter 5: Salmonella, ISO 6579—2017 Microbiology of the food chain-Horizontal method for the detection, enumeration and serotyping of Salmonella and GB 4789.4—2016 National standard for food safety-Food microbiology testing Salmonella testing were compared through parallel experiments with 20 different serotypes of Salmonella. Different artificial contaminated food matrices (infant formula milk powder, fermented milk products samples and environmental samples) were used in the experiments to evaluate the applicability of the 3 kinds of standard methods. Results The detection limits of the methods of FDA/BAM, ISO 6579—2017, and GB 4789.4—2016 were between 10-1?100 CFU/sample. At the contamination level of 101 CFU/sample, the sensitivities of the 3 kinds of standard methods were all 100%; The sensitivities were 95%, 90% and 90% respectively for the 3 kinds of standard methods at the contamination level of 100 CFU/sample; And the sensitivities were 65%, 50% and 45% respectively at the contamination level of 10-1 CFU/sample. For the 3 types of artificial contaminated samples, the detection limit of FDA/BAM method was significantly higher than that of ISO 6579—2017 and GB 4789.4—2016 (P<0.05). And there was no significant difference between the methods of ISO 6579—2017 and GB4789.4—2016 (P>0.05). Conclusion The 3 kinds of standard methods have nearly the same detection limits and are all effective methods for detecting Salmonella in food. The sensitivity of FDA/BAM method is higher than those of ISO 6579—2017 and GB4789.4—2016 at the contamination levels of 10-1?100 CFU/sample. In different artificially contaminated Salmonella food matrices, the detection rate of FDA/BAM method is significantly higher than those of ISO 6579—2017 and GB4789.4—2016.