辽宁省市售水产品中副溶血性弧菌的污染状况及耐药性分析

Analysis of contamination and drug resistance of Vibrio parahaemolyticus in aquatic products sold in Liaoning province

中文关键词:  水产品  副溶血性弧菌  毒力基因  耐药性  基质辅助激光解吸-电离飞行时间质谱法

英文关键词:aquatic products  Vibrio parahaemolyticus  virulence gene  antimicrobial resistance  matrix-assisted laser desorption/ionization time of flight mass spectrometry

中文摘要:

      目的 分析辽宁省市售水产品副溶血性弧菌污染状况、毒力基因分布及耐药情况。方法 采集420份水产品, 应用国标方法对水产品中副溶血性弧菌进行分离和鉴定, 同时应用基质辅助激光解吸-电离飞行时间质谱法(matrix-assisted laser desorption/ionization time of flight mass spectrometry, MALDI-TOF MS)对鉴定结果进行验证。分析不同种类、不同类别、不同季节、不同地区水产品中副溶血性弧菌检出情况, 同时对分离菌株的毒力基因及耐药性进行检测。结果 经分离鉴定得到123株副溶血性弧菌, 总检出率为29.29% (123/420)。应用MALDI-TOF MS技术对副溶血性弧菌鉴定, 其结果与国标方法鉴定结果一致。分析发现, 海水产品检出率为33.44%; 海水贝类检出率最高为49.51%, 其中淡水产品中河蟹检出率较高为61.29%; 第三季度检出率最高为39.86%; 营口检出率最高为41.89%。分离的123株菌tlh基因全部阳性, 未检测到tdh和trh基因, 对氨苄西林耐药性显著, 耐药率为94.31%。结论 辽宁省市售水产品不同程度受到副溶血性弧菌污染, 并呈现一定程度的耐药性, 污染尤其以河蟹风险最高, 应引起相关部门的重视, 及时采取有效措施预防由水产品引起的副溶血性弧菌食源性疾病。

英文摘要:

      Objective To analyze the contamination level, distribution of virulence gene and drug resistance of Vibrio parahaemolyticus in aquatic products in the retail markets of Liaoning province. Methods Vibrio parahaemolyticus isolated from 420 aquatic products sold in Liaoning province was identified by national standard method, and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) was used to verify the identification results. The contamination of Vibrio parahaemolyticus in different kinds, different categories, different seasons, different areas of aquatic products, virulence genes and drug resistance were simultaneously analyzed. Results Total of 123 strains of Vibrio parahaemolyticus were authenticated, and the detection rate was 29.29% (123/420). The identification results of MALDI-TOF MS were consistent with the national standard method. The detection rate of Vibrio parahaemolyticus in seafood was 33.44%, and the detection rate in shellfish was the highest (49.51%). In freshwater products, the detection rate of freshwater crab was the highest (61.29%). The detection rate in the third quarter was highest (39.86%), and the detection rate in Yingkou was highest (41.89%). The tlh gene was detected in all strains, however, tdh and trh genes were not detected in all strains. Most of the strains were resistant to ampicillin (94.31%). Conclusion The aquatic products sold in Liaoning province were polluted by Vibrio parahaemolyticus in different levels, and showed a degree of drug resistance, most of them resisted ampicillin, and especially freshwater crabs were particularly at risk from pollution. More attention should be paid and effective measures should be taken to prevent food borne diseases caused by Vibrio parahaemolyticus.