绍兴市生食鱼中副溶血性弧菌毒力基因及耐药性与分子分型研究

Study on virulence gene, drug resistance, molecular typing of Vibrio parahaemolyticus in raw fish in Shaoxing

中文关键词:  生食鱼  副溶血性弧菌  毒力基因  药敏试验  分子分型

英文关键词:raw fish  Vibrio parahaemolyticus  virulence gene  drug sensitivity test  molecular typing

中文摘要:

      目的 了解绍兴市生食鱼中副溶血性弧菌的污染状况、毒力基因携带情况、耐药性及分子分型情况。方法 采集绍兴地区354份生食鱼样品, 参照GB 4789.7—2013《食品安全国家标准 食品微生物学检验 副溶血性弧菌检验》进行菌株的分离与鉴定; 利用荧光定量聚合酶链反应技术对分离株进行毒力基因检测; 采用微量肉汤稀释法和脉冲场凝胶电泳法对分离菌株分别进行药物敏感试验和分子分型。结果 354份生食鱼中有13份样品被检出副溶血性弧菌, 总检出率为3.7%。13株菌全部检出tlh基因, tdh和trh基因均未检出。13株菌对头孢唑林均耐药, 3株菌对氨苄西林耐药, 对其他抗生素均敏感。脉冲场凝胶电泳条带分散, 显示遗传特征多样。结论 绍兴市生食鱼中存在一定程度的副溶血性弧菌污染, 菌株毒力基因携带率低, 对头孢唑林普遍耐药, 遗传特征多样。

英文摘要:

      Objective To investigate the contamination status, virulence gene carrying status, drug resistance and molecular typing of Vibrio parahaemolyticus in raw fish in Shaoxing. Methods A total of 354 raw fish samples were collected in Shaoxing area. The strains were isolated and identified according to GB 4789.7—2013 National food safety standard-Microbiological examination of food-Vibrio parahaemolyticus. Fluorescence quantitative polymerase chain reaction was used to detect virulence genes of the isolates. Microbroth dilution method and pulsed field gel electrophoresis were used for drug sensitivity test and molecular typing of the isolates, respectively. Results Vibrio parahaemolyticus was detected in 13 samples of 354 raw fish, with a total detection rate was 3.7%. All the 13 strains were positive for tlh gene and negative for tdh, trh gene. All the strains were resistant to cefazolin, 3 strains were resistant to ampicillin, and all were sensitive to other antibiotics. The pulsed field gel electrophoresis bands were dispersed and the genetic characteristics were diverse. Conclusion There is a degree of contamination of Vibrio parahaemolyticus in raw fish in Shaoxing, the carrying rate of virulence genes of strains is low, strains are resistant to cefazolin and the genetic characteristics are diverse.