山西葡萄中链格孢菌分离鉴定与污染水平分析

Isolation and identification and analysis of pollution level of Alternaria

中文关键词:  病原微生物  链格孢菌  山西葡萄

英文关键词:pathogenic microorganism  Alternaria alternata  Shanxi grape

中文摘要:

      目的 初步明确山西葡萄(巨峰葡萄和红提葡萄)中链格孢菌的污染水平。方法 本研究对山西葡萄主产区的巨峰葡萄和红提葡萄上的链格孢菌进行纯化分离、真菌形态学鉴定、分子生物学鉴定, 并对链格孢菌产毒能力进行检测分析。结果 链格孢菌(Alternaria alternata)侵染山西葡萄具有品种选择性, 即较巨峰葡萄, 链格孢菌更易侵染红提葡萄。链格孢菌菌株产生的毒素主要以细交链孢菌酮酸(tenuazonic acid, TeA)为主, TeA的检出量占总体检出毒素量的98.14%。结论 本研究分离得到山西葡萄中的链格孢菌易侵染红提葡萄, 且产生毒素以TeA为主。基于链格孢毒素的毒性、污染范围及对人体的潜在危害, 应加快建立链格孢霉毒素的限量标准, 同时加强葡萄及其制品的风险监测。

英文摘要:

      Objective To determine the pollution level of Alternaria alternata in Shanxi grape (kyoho grapes and redglobe table grapes) preliminarily. Methods Alternaria alternata isolated from kyoho grapes and redglobe table grapes in the main grape producing area of Shanxi province were identified by purification, morphology and molecular biology, and the toxin-producing ability of Alternaria alternate was analyzed. Results The infection of Alternaria alternata to grape varieties was selective in Shanxi province, which mesns the infection of Alternaria alternata to redglobe table grapes was more serious than kyoho grapes. The toxin produced by Alternaria alternata was mainly TeA, the content of TeA was 98.14% of the total detected toxin. Conclusion Alternaria alternate isolated from Shanxi grape is easy to infect redglobe table grapes and produced TeA toxin mainly. Based on the toxicity, pollution range and potential harm to human body, it is necessary to set up the limit standard for Alternaria alternata toxin and strengthen the risk monitoring of grape and its products.