快速检测羟自由基的试纸开发

Development of the test paper for rapid detection of hydroxyl radical

中文关键词:  抗羟自由基  抗氧化活性  试纸法  快速检测

英文关键词:antihydroxyl radical  antioxidation activity  paper method  rapid detection

中文摘要:

      目的 开发用试纸条检测羟自由基(?OH)的方法, 并用该方法检测具有抗氧化活性的物质。方法 利用Fenton反应的原理, 优化羟基萘酚蓝(hydroxynaphthol blue, HNB)浓度、显色剂浓度、pH、金属离子和H2O2等条件, 并测定产生的羟自由基产生量, 通过观察试纸的颜色变化检测H2O2含量。结果 最佳试纸条件为: Mn2+的浓度是2×10?6 mol/L、pH 10.5的NaOH-KHCO3缓冲溶液、HNB浓度为5×10?3 mol/L、H2O2溶液浓度为0.15 mol/L。以硫尿、甘露醇为对照, 优化试纸条件检测H2O2, 验证其可以检测具有抗氧化活性的物质。结论 所建立的试纸检测方法简单、方便、快速, 可用于现场抗氧化物质清除率羟自由基(?OH)活性的快速检测方法。

英文摘要:

      Objective To develop a method of detecting hydroxyl radical with test paper, and to detect the antioxidant by this test paper. Methods The conditions of hydroxynaphthol blue (HNB) concentration, developer concentration, pH, metal ion and H2O2 were optimized, the amount of hydroxyl free radical produced was determined, and the amount of H2O2 would be detected by the color change of the test paper. Results The optimal test paper conditions were as follows: the concentration of Mn2+ was 2×10?6 mol/L, the pH value was 10.5 (NaOH-KHCO3 buffer solution), the concentration of HNB was 5×10?3 mol/L, and the concentration of H2O2 solution was 0.15 mol/L. Compared with thiourea and mannitol, H2O2 was detected by optimizing the test paper, and the results showed this test paper could be used to detect the antioxidant with the active ability of hydroxyl radical. Conclusion This method is simple, convenient and rapid, which can be used for the rapid detection of hydroxyl radical activity.