高效液相色谱法测定保健食品中的虾青素含量

Determination of astaxanthin in functional food by high performance liquid chromatography

中文关键词:  虾青素  高效液相色谱法  保健食品

英文关键词:astaxanthin  high performance liquid chromatography  functional food

中文摘要:

      目的 建立高效液相色谱法检测保健食品中虾青素含量的分析方法。方法 采用油状形态和粉末状形态样品来源的保健食品, 样品经二氯甲烷-甲醇混合溶液(1:3, V:V)提取、NaOH甲醇溶液皂化后, 经高效液相色谱分离、紫外可见光检测器检测。结果 全反式虾青素在0.25~12.70 μg/mL范围内呈现良好的线性关系, 检出限为1.09 ng/mL, 定量限为3.64 ng/mL。油状形态样品虾青素的精密度为2.4%、回收率为96.7%。粉末形态样品虾青素的精密度为2.0%、回收率为98.6%。在21.9 mmol/L以下浓度范围内NaOH不会造成虾青素的降解。结论 该方法回收率高、精密度良好, 测量结果准确, 可以应用于保健食品中虾青素含量的测定。

英文摘要:

      Objective To establish a method for the determination of astaxanthin in health food by high performance liquid chromatography. Methods Sample sources of health foods in oil form and powder form were used. The samples were extracted by dichloromethane methanol mixed solution (1:3, V:V) and saponified with NaOH methanol solution. The samples were separated by high performance liquid chromatography and detected by ultraviolet-visible detector. Results All trans astaxanthin showed good linear relationship in the range of 0.25?12.70 μg/mL, the limit of detection was 1.09 ng/mL, and the limit of quantification was 3.64 ng/mL. The precision and recovery of astaxanthin in oil sample were 2.4% and 96.7%, respectively. The precision and recovery of astaxanthin in powder sample were 2.0% and 98.6%, respectively. NaOH did not degrade astaxanthin in the concentration range below 21.9 mmol/L. Conclusion This methodhas high recovery rate, good precision and accurate measurement results, and is suitable for the determination of astaxanthin in healthy food.