铁皮石斛对脾虚便秘小鼠肠道形态及肠道免疫细胞数量的影响

Effects of Dendrobium officinalis on intestinal morphology and number of intestinal immune cells in mice with spleen-deficiency and constipation

中文关键词:  铁皮石斛  脾虚便秘  绒毛高度  隐窝深度  黏膜厚度  淋巴细胞数

英文关键词:Dendrobium candidum  spleen-deficiency constipation  villus height  crypt depth  mucosal thickness  lymphocyte number

中文摘要:

      目的 探究铁皮石斛对脾虚便秘小鼠的肠隐窝深度、黏膜厚度、绒毛高度及淋巴细胞数的影响, 从肠道形态和肠道免疫明确铁皮石斛对脾虚便秘的疗效机制。方法 18只SPF级KM小鼠随机分为正常组、模型组和治疗组。通过灌胃番泻叶水煎液7 d, 控制饮食、饥饱失常8 d建立小鼠脾虚便秘模型。造模成功后, 治疗组灌胃铁皮石斛汤剂, 正常组与模型组灌胃等量无菌水, 持续5 d。实验结束后, 采集各组小鼠回肠、空肠和盲肠约2 cm肠段, 分析隐窝深度、绒毛高度、黏膜厚度和淋巴细胞数。结果 与正常组和模型组相比, 铁皮石斛能增加小鼠空肠淋巴细胞数和回肠肠黏膜厚度(P<0.01或P<0.05)。治疗组小鼠盲肠绒毛高度显著高于正常组(P<0.05), 回肠绒毛高度虽然高于正常组, 但差异不显著(P>0.05), 治疗组小鼠盲肠黏膜厚度和淋巴细胞数高于模型组, 二者差异显著(P<0.05)。结论 铁皮石斛通过改善脾虚便秘小鼠肠道形态, 增加黏膜淋巴细胞数来治疗脾虚便秘。

英文摘要:

      Objective To investigate the effects of Dendrobium candidum on intestinal crypt depth, mucosal thickness, villi eight and lymphocyte number in mice with spleen-deficiency and constipation, and to clarify the therapeutic effect of morphology and intestinal immunity. Methods A total of 18 KM mice (SPF) were randomly divided into model group, normal group and treatment group. The constipation mice with spleen-deficiency were fed with Seine decoction for 7 d and restricted diet for 8 d, so as to cause their hunger and satiety disorder. After successful modeling, the treatment group was given Dendrobium candidum decoction by gavage for 5 d. The normal group and the model group were given the same amount of sterile water. After the experiment, the ileum, jejunum and cecum segments of each group were collected about 2 cm each, and the crypt depth, villi height, mucosal thickness and lymphocyte number were measured. Results Dendrobium candidum significantly increased the number of jejunum lymphocytes and ileum intestinal mucosa thickness in mice, with significant differences compared with the normal group and model group (P<0.01 or P<0.05). The height of the cecal villi in the treatment group was significantly higher than that in the normal group (P<0.05), and the height of the ileal villi was higher than the normal group, but there was no statistically significant difference (P>0.05). Meanwhile, the thickness of the cecal mucosa and the number of lymphocytes in the treatment group were significantly higher than that in the model group (P<0.05). Conclusion Dendrobium candidum can significantly improve the intestinal morphology of mice with spleen-deficiency and constipation, and can increase the number of mucosal lymphocytes, which is related to the treatment of spleen-deficiency and constipation.