一种无溶剂饮水舱防腐涂料的毒性研究

Toxicity of a solvent-free anticorrosive coating for drinking water tank

中文关键词:  无溶剂  防腐涂料  急性毒性  微核  Ames实验

英文关键词:solvent-free  anticorrosive coating  acute toxicity  micronucleus  Ames test

中文摘要:

      目的 探讨一种无溶剂饮水舱防腐涂料的毒性作用。方法 急性经口毒性实验采用啮齿类动物急性固定剂量经口毒性实验一次限量法, 剂量设为20000 mg/(kg BW)。微核实验设5000、2500、1250 mg/(kg BW) 3个剂量组、阴性对照组及阳性对照组, 采用30 h给受试物法, 检测骨髓嗜多染红细胞微核率。Ames实验分别设每皿200、100、40、10 μL共4个剂量组, 同时设阴性、阳性对照组, 采用平板掺入法, 观察每皿回变菌落数。结果 该种无溶剂饮水舱防腐涂料对受试动物的急性毒性(median lethal dose, LD50)＞20000 mg/(kg BW)。与阴性对照组比较, 骨髓细胞微核试验结果为阴性。三七须根各剂量组在Ames实验中未呈现致突变性。 结论 在本实验条件和剂量范围内, 该种无溶剂饮水舱防腐涂料对昆明种小鼠的急性经口毒性属实际无毒, 未见诱导小鼠骨髓嗜多染红细胞微核形成的能力, 在Ames实验中未见呈现致突变作用。

英文摘要:

      Objective To investigate the toxicity of a solvent-free anticorrosive coating for drinking water tank. Methods The acute oral toxicity test was carried out in rodents at a fixed dose of 20000 mg/(kg BW). The bone marrow cells micronucleus test was divided into three dose groups: 5000, 2500, 1250 mg/(kg BW), negative control group and positive control group. The micronucleus rate of bone marrow polychromatic erythrocytes was detected by 30 h test. Ames test was divided into four dose groups: 200, 100, 40 and 10 μL per dish, and negative and positive control groups were set up at the same time. Plate incorporation method was used to observe the number of revertant colonies in each dish. Results The median lethal dose (LD50) of the solvent-free anticorrosive coating for drinking water tank to the tested animals was more than 20000 mg/(kg BW). Compared with the negative control group, the bone marrow cells micronucleus test results were negative. No mutagenicity of Panax notoginseng root was observed in each dose group in the Ames test. Conclusion Within the experimental conditions and dosage range, the solvent-free anticorrosion coating for drinking water tank is practically non-toxic to Kunming mice, and there is no ability to induce micronucleus formation of polychromatic erythrocytes in bone marrow of mice, and there is no mutagenic effect in Ames test.