自动微流控膜芯片法检测副溶血性弧菌

Detection of Vibrio parahaemolyticus by auto-microfluidic thin-film chip method

中文关键词:  副溶血性弧菌  自动微流控  膜芯片

英文关键词:Vibrio parahaemolyticus  auto-microfluidic  thin-film chip

中文摘要:

      目的 建立自动微流控膜芯片检测法副溶血性弧菌的方法。方法 利用自动微流控膜芯片对副溶血性弧菌进行检测, 并采用行业标准SN/T 2424-2010实时荧光PCR方法进行比对验证。结果 该技术具备副溶血性弧菌的检测能力, 验证实际样品的检测结果与行业标准荧光PCR法一致。结论 该方法快速、准确、直观, 适合测定副溶血性弧菌。

英文摘要:

      Objective To establish a method for the detection of Vibrio parahaemolyticus by auto-microfluidic thin-film chip technology. Methods Vibrio parahaemolyticus were detected by auto-microfluidic thin-film chip, and the real-time fluorescent PCR method SN/T 2424-2010 required by industry standard was used to verify the results. Results The technology had the ability to identify Vibrio parahaemolyticus. The results of comparison and verification of actual samples were consistent with those of industry standard flrorescent PCR. Conclusion The technology is rapid, accurate and intuitive, which is suitable for detecting Vibrio parahaemolyticus.