食叶草粉对SD大鼠的致畸性研究

Study on the teratogenicity of edible dock powder on SD rats

中文关键词:  食叶草  致畸性  胚胎  母体毒性  大鼠

英文关键词:edible dock  teratogenicity  embryos  maternal toxicity  rat

中文摘要:

      目的 探究食叶草粉对SD大鼠的母体毒性、胚胎毒性和致畸性。方法 受孕SD大鼠按体重随机分组, 每组不少于16只, 设溶剂对照组(蒸馏水)和食叶草粉低、中、高剂量组(分别为0.42、0.84、1.67 g/kg.BW)。在受孕第6~15 d, 每日以10 mL/kg.BW灌胃给予受试物或等量蒸馏水。观察、记录孕鼠临床表现和体重, 于孕期第20 d称重并处死母鼠, 检查孕鼠妊娠情况和胎鼠发育状况, 包括黄体数、死胎数、吸收胎数、活胎数, 胎仔性别、体重、体长、尾长, 观察有无骨骼畸形和内脏畸形。结果 将各剂量组的各项指标与溶剂对照组指标进行比较, 差异无统计学意义(P>0.05)。结论 食叶草粉在本实验条件下对SD大鼠无母体毒性、胚胎毒性和致畸性。

英文摘要:

      Objective To explore the maternal toxicity, embryo toxicity and teratogenicity of edible dock powder in SD rats. Methods Pregnant SD rats were randomly divided into groups according to body weight, no less than 16 rats in each group. Solvent control group (distilled water) and low, medium and high dose groups of grass meal (0.42, 0.84 and 1.67 g/kg.BW, respectively) were set. During the 6th to the 15th day of gestation, the maternal rats were orally given distilled water or edible dock power at 10 mL/ kg.BW once daily, respectively. In the course of the study, clinical manifestations and body weights of the pregnant rats were observed and recorded weekly. The pregnant rats were weighted and humanely sacrificed on the 20th day in pregnancy. Pregnancy of rats and fetal development were examined, including the numbers of corpus luteum, died fetuses, absorbed fetuses and living fetuses, the gender, body weight, body length, tail length and deformity of the fetal rats. Results There was no significant difference between the indexes of each dose group and those of the solvent control group (P>0.05). Conclusion Edible dock powder has no maternal toxicity, embryo toxicity and teratogenicity to SD rats under the experimental conditions