玫瑰花醇提部位荧光特性研究

Study on fluorescence characteristics of ethanol extract from rosae rugosae flos

中文关键词:  玫瑰花  三维荧光光谱  荧光特性  质量评价

英文关键词:rosae rugosae flos  three-dimensional fluorescence spectrum  fluorescence characteristics  quality evaluation

中文摘要:

      目的 建立荧光光谱法测定玫瑰花醇提部位荧光特性的研究方法。方法 采用荧光分析法结合紫外光谱测定玫瑰花醇提部位的光谱特征, 并对浓度、温度以及放置时间等因素对荧光特性的影响进行研究。 结果 玫瑰醇提部位在Ex 287 nm/Em 364 nm存在一荧光特征峰, 其荧光强度受浓度影响较大, 在0.1~ 0.4 mg/mL浓度范围内, 荧光强度随浓度的增大而增强; 玫瑰花醇提部位荧光强度随温度的升高而降低; 此外, 在48 h内, 其荧光强度较为稳定。结论 玫瑰花醇提部位存在明显的荧光特征峰, 且甘肃、山东及贵州3个不同产地荧光特征峰在强度上存在一定差异, 为进一步完善玫瑰花药材质量评价系统提供了理论基础。。

英文摘要:

      Objective To establish a method for determining the fluorescence characteristics of rosae rugosae flos ethanol extract by fluorescence spectrometry. Methods The spectral characteristics of rosae rugosae flos ethanol extract were determined by fluorescence analysis combined with ultraviolet spectroscopy, and the effects of concentration, temperature and placing time on the fluorescence characteristics were studied. Results There was a characteristic fluorescence peak at Ex 287 nm/Em 364 nm at the ethanol extract site of rosae rugosae flos. The fluorescence intensity was greatly affected by the concentration. In the concentration range of 0.1–0.4 mg/mL, the fluorescence intensity was enhanced with the increase of concentration. The fluorescence intensity of rosae rugosae flos ethanol extract decreased with the increase of temperature. In addition, the fluorescence intensity was relatively stable within 48 h. Conclusion There are significant fluorescence characteristic peaks in the ethanol extract part of rosae rugosae flos, and there are some differences in fluorescence characteristic peaks from 3 different origins, Gansu, Shandong and Guizhou. It provides a theoretical basis for further improving the quality evaluation system of rose.