硬糖类产品中桉叶素的含量测定

Determination of the content of cineole in hard candy

中文关键词:  桉叶素  硬糖  检测方法

英文关键词:cineole  hard candy  detection method

中文摘要:

      目的 建立适用于硬糖类产品中桉叶素的检测方法。方法 采用气相色谱法测定硬糖中桉叶素的含量。采用Agilent HP-INNOWAX毛细管色谱柱(柱长为30 m, 内径为0.32 mm, 膜厚为0.25 μm), 采用顶空进样, 进样量为5 mL, 炉温为85 ℃, 平衡时间为30 min, 进样口温度为150℃, 柱温为程序升温: 初始温度70 ℃, 以5 ℃/min的升温速率升温至125 ℃, 保持2 min, 再以20 ℃/min的升温速率升温至200 ℃, 保留 5 min; 检测器温度为180 ℃, 载气流速为1.0 mL/min。结果 桉叶素回归方程Y=8622.2X-32.028, 相关系数r2为0.9998, 线性范围为0.00284~0.56720 mg/mL, 加样回收率为104.31%。结论 该法简便、准确、专属性强、重复性好, 经方法学验证, 本法可用于硬糖类产品中桉叶素的含量测定。

英文摘要:

      Objective To establish a method for the determination of cineole inhard candy. Methods The content of eucalyptus in hard candy was determined by gas chromatography. Agilent HP-INNOWAXCapillary chromatographic column (30 m×0.32 mm, 0.25 μm)was used. Headspace sample was used for injection, the amount of sample was 5 mL, the furnace temperature was 85 ℃, the equilibrium time was 30 min, and the inlet temperature was 150 ℃.The column temperature was programmed: the initial temperature was 70 ℃, and the temperature was increased to 125 ℃ at a rate of 5 ℃/min for 2 min. Then the temperature was increased to 200 ℃ at a rate of 20 ℃/min for 5 min. The detector temperature was 180 ℃, and the flow rate of carrier gas was 1.0 mL/min. Results The regression equation was Y=8622.2X?32.028, the correlation coefficient r2was 0.9998, the linear range were 0.00284?0.56720 mg/mL, and the recovery was 104.31%. Conclusion This method is simple, accurate, specific, and reproducible. It has been verified by methodology that this method can be used to determine the content of cineole in hard candy products.