实时荧光PCR法和国标法检测乳粉中单增李斯特菌的比较研究

Comparative study for detection of Listeria monocytogenes by real-time fluorescent polymerase chain reaction and national standard method in milk powder

中文关键词:  乳粉  单增李斯特菌  实时荧光PCR法

英文关键词:milk powder  Listeria monocytogenes  real-time fluorescent polymerase chain reaction

中文摘要:

      目的 探究实时荧光PCR法是否可用于实验室乳粉中单增李斯特菌的检测。方法 依据GB 4789.30-2016《食品安全国家标准 食品微生物学检验 单核细胞增生李斯特氏菌检验》和能力验证作业指导书对乳粉中单增李斯特菌进行检测。利用实时荧光PCR法(real-time fluorescent polymerase chain reaction, RT-PCR)对能力验证中的2个样品进行快速检测, 同时采用VITEK2 Compact30全自动微生物鉴定系统对国标法中挑取的可疑菌落进行鉴定, 比较国标法和RT-PCR法的检测结果。结果 RT-PCR法和国标法对2个样品的检测结果一致, 即样品CODE: FC02220028检出单增李斯特菌, 样品CODE: FC02220098未检出单增李斯特菌。结论 RT-PCR法可用于实验室乳粉中单增李斯特菌的检测。

英文摘要:

      Objective To explore whether real-time fluorescent polymerase chain reaction(RT-PCR) method can be used to detect Listeria monocytogenes from milk powder in laboratory. Methods Listeria monocytogenes testing was carried out according to GB 4789.30-2016 National food safety standard-Food microbiological examination-Listeria monocytogenes and ability verification guidance book. RT-PCR method was used for testing the 2 samples, and suspicious strains of 2 samples were identified by automatic microbial identification system VITEK2 Compact 30. Then the results of national standard method and RT-PCR method were compared. Results Both national standard method and RT-PCR method proved that Listeria monocytogenes was detected in CODE: FC02220028, not detected in CODE: FC02220098. Conclusion RT-PCR method can be used to detect Listeria monocytogenes from milk powder in laboratory milk power.