DNA检测技术在鳕鱼及其制品鉴定中的应用

Application of DNA-based methods for the identification of cod fish and its products

中文关键词:  鳕鱼  成分鉴定  限制性片段长度多态性聚合酶链反应技术  DNA条形码技术  环介导等温扩增技术  实时荧光定量PCR技术

英文关键词:cod fish  identification of constituents  cleaved amplification polymorphism sequence-tagged sites  DNA barcoding  loop-mediated isothermal amplification  quantitative real-time PCR

中文摘要:

      鳕鱼是经济价值高和营养价值高的重要食用鱼类, 但由于市场上鱼类俗名混乱问题, 以及不同鱼种之间价值的巨大差异, 错误标识鳕鱼产品或以次充好的现象时有发生, 有可能因为含有过敏原成分或有毒成分造成食品安全风险。因此, 需要快速可靠的鳕鱼成分鉴定方法和来保障市场监管。目前, 鳕鱼及其制品的鉴定方法主要为DNA检测技术。本文主要介绍了DNA指纹图谱限制性片段长度多态性聚合酶链反应(cleaved amplification polymorphism sequence-tagged sites, PCR-RFLP)技术、DNA条形码技术、环介导等温扩增技术和实时荧光定量PCR技术的原理、优缺点、在鳕鱼及其制品鉴定中的应用, 最后讨论了鳕鱼及其制品鉴定方法的发展趋势和监管建议。

英文摘要:

      Cod fish is an important edible fish with high economic value and high nutritional value. However, due to the confusion of common names of fish in the market and the huge difference in value between different fish species, there are some reports of cod fish products mislabeling or selling seconds at best quality price, and food safety risk may be caused by allergen or toxic ingredients. Therefore, fast and reliable identification methods are needed to ensure market supervision. At present, DNA-based methods are mainly identification methods of cod fish and products. This paper introduced the principles, advantages and disadvantages of cleaved amplification polymorphism sequence-tagged sites (PCR-RFLP), DNA barcoding, loop-mediated isothermal amplification and quantitative real-time PCR in cod fish and products species identification. Finally, the perspectives and supervisory suggestion of this field were discussed.