腹泻患儿粪便中沙门菌的血清型、药敏分析及脉冲场凝胶电泳分子分型研究

Serotype, drug resistance analysis and pulsed field gel electrophoresis molecular typing of Salmonella isolated from feces of children with diarrhea

中文关键词:  食源性疾患  沙门菌  血清型  药敏实验  脉冲场凝胶电泳

英文关键词:foodborne diseases  Salmonella  serotype  drug sensitivity test  pulsed field gel electrophoresis

中文摘要:

      目的 研究2019年北京市朝阳区0~10岁腹泻患儿粪便中分离出的沙门菌的血清型、脉冲场凝胶电泳(pulsed field gel electrophoresis, PFGE)分子分型研究及耐药特点。方法 对分离自腹泻患儿病例的47株沙门菌进行血清分型, 采用微量肉汤稀释法进行27种抗生素的药敏实验; 采用PFGE脉冲场凝胶电泳进行指纹图谱分型研究。结果 47株沙门分为8种血清型, 优势血清型有2种, 分别为肠炎沙门菌23株占48.94%, 鼠伤寒沙门菌14株占29.79%。47株沙门菌对磺胺异恶唑耐药率(57.45%)最高, 其次为氨苄西林(48.94%)和链霉素(48.94%)。23株肠炎沙门菌可分成8个PFGE指纹图谱, 14株鼠伤寒沙门菌可分成13个PFGE指纹图谱。结论 北京市朝阳区0~10岁儿童食源性沙门菌血清主要为肠炎沙门菌和鼠伤寒沙门菌, 各血清型的耐药性有所不同, PFGE指纹图谱呈多样性。

英文摘要:

      Objective To study the serotype, pulsed field gel electrophoresis(PFGE) molecular typing and drug resistance characteristics of Salmonella isolated from feces of children aged 0?10 years with diarrhea in Chaoyang district of Beijing in 2019. Methods The serotyping of 47 Salmonella isolated from children with diarrhea was performed. The sensitivity of 27 antibiotics was determined by micro broth dilution method. The fingerprints were classified by PFGE. Results The 47 Salmonella strains were divided into8 serotypes, of which the dominant serotypes were 2, including 23 strains of Salmonella enteritis (48.94%) and14 strains of Salmonella typhimurium (29.79%). The drug resistance rate of 47 Salmonella strains to sulfamethoxazole (57.45%) was the highest, followed by ampicillin (48.94%) and streptomycin (48.94%), and 23 strains of Salmonella enteritidis could be divided into 8 PFGE fingerprints, and 14 Salmonella typhimurium strains could be divided into 13 PFGE fingerprints. Conclusion Salmonella enteritidis and Salmonella typhimurium are the main food borne Salmonella in children aged 0?10 years in Chaoyang district of Beijing. The drug resistance of each serotype was different, and the PFGE fingerprint is diverse.