离子色谱法测定茶园土壤中草甘膦残留量

Determination of glyphosate residues in tea plantation soil by ion chromatography

中文关键词:  离子色谱法  草甘膦  残留量  茶园土壤

英文关键词:ion-chromatography  glyphosate  pesticide residues  tealand soil

中文摘要:

      目的 建立简便快捷测定茶园土壤草甘膦残留量的离子色谱分析方法。方法 土壤样品采用5 mg/L Na2S2O3超声提取30 min, 提取液经净化后, 采用流速为1.0 mL/min、进样量25 ?L、抑制器电流87 mA、 20 mmol/L KOH淋洗液、AS19分析柱, 抑制型电导检测。结果 草甘膦在0.05~3.0 mg/L范围内线性关系良好, 相关系数r为0.9996, 检出限为0.016 mg/kg。在低、中、高3种加标水平下, 平均回收率分别为90.4%、96.8%和97.8%, 重复测定相对标准偏差(RSD)分别为5.73%、7.74%和3.87%。结论 该方法操作简单无需衍生, 线性良好, 可以准确地测定茶园土壤中草甘膦残留量。

英文摘要:

      Objective To establish a simple and rapid ion chromatography analysis method for the determination of glyphosate residues in tea garden soil. Methods The soil samples were extracted with 5 mg/L Na2S2O3 for 30 min. After purification, the flow rate was 1.0 mL/min, injection volume was 25 ?L, inhibitor current was 87 mA, 20 mmol/L KOH eluent, AS19 analytical column and inhibitory conductance detection was used. Results Glyphosate had a good linear relationship in the range of 0.05–3.0 mg/L, the correlation coefficient r was 0.9996, and the detection limit was 0.016 mg/kg. The average recoveries of glyphosate were 90.4%, 96.8% and 97.8%, respectively, and the RSD% of repeated determination were 5.73%, 7.74% and 3.87%, respectively. Conclusion The method is simple to operate, does not need derivation, has good linearity, and can accurately determine glyphosate residues in tea garden soil.