高效液相色谱法同时测定磺胺喹噁啉二甲氧苄啶预混剂中两种主要成分含量

Simultaneous determination of 2 main components in sulfaquinoxoline dimethoprim premix by high performance liquid chromatography

中文关键词:  磺胺喹噁啉  二甲氧苄啶  高效液相色谱法

英文关键词:sulfaquinoxaline  diaveridine  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)同时测定磺胺喹噁啉二甲氧苄啶预混剂中磺胺喹噁啉和二甲氧苄啶含量的分析方法。方法 采用Waters Symmetry C18分析柱(250 mm ×4.6 mm, 5 μm), 以0.1%磷酸―乙腈(75:25)为流动相, 流速为1.0 mL/min, 检测波长为240 nm, 采用外标法进行定量。结果 在此色谱条件下, 2种组分分离度良好, 分别在5.0~100 μg/mL、1.0~20 μg/mL范围内线性关系良好(r2=0.99998); 磺胺喹噁啉按0.2 mg/mg添加, 平均回收率为97.86%(RSD=0.8%), 二甲氧苄啶按 0.04 mg/mg添加, 平均回收率95.14%(RSD=1.9%)。结论 该方法简便、快速、准确, 重复性良好, 可用于磺胺喹噁啉二甲氧苄啶预混剂中磺胺喹噁啉和二甲氧苄啶的同时测定。

英文摘要:

      Objective To establish a method for the simultaneous determination of sulfaquinoxaline and dimethoxidine in sulfaquinoxaline dimethoxidine premix by high performance liquid chromatography (HPLC). Methods Analysis was performed on a Waters Symmetry C18 (250 mm×4.6 mm, 5 μm). The mobile phase was considered of 0.1% phosphoric acid solution and acetonitrile (75:25), with a flow rate of 1.0 mL/min and wavelength of 240 nm. It was quantified by external standard method. Results Two substances were separated effectively, sulfaquinoxaline and diaveridine had good linearity over the range of 5.0-100 μg/mL and 1.0-20 μg/mL, respectively(r2=0.99998). Sulfaquinoxaline was added at 0.2 mg/mg, with an average recovery of 97.86% (RSD=0.8%). Dimethoxybenzidine was added at 0.04 mg/mg, with an average recovery of 95.14% (RSD=1.9%). Conclusion This method is accurate, rapid and repeatable, which can be used for the simultaneous determination of sulfaquinoxaline and diaveridine in sulfaquinoxaline and diaveridine premix.