响应面法优化天山岩黄芪多糖提取工艺

Optimization of extraction technology of polysaccharides from Hedysarum semenovii Regelet Herd by response surface methodology

中文关键词:  多糖  提取工艺  响应面分析法

英文关键词:polysaccharides  extraction process  response surface analysis

中文摘要:

      目的 优化天山岩黄芪中多糖的提取工艺。方法 以多糖含量作为评价指标, 以提取时间、提取温度、液料比为考察因素, 采用单因素试验与响应面分析相结合的方法, 探讨适宜的提取工艺。结果 天山岩黄芪多糖的最佳提取工艺条件为: 提取料液比1:32(m:V)、提取时间3 h、提取温度92 ℃。在此条件下, 多糖含量的实际验证含量为6.33%, 与预测值6.39%接近。结论 所选工艺合理、可行, 可用于天山岩黄芪中多糖的提取。

英文摘要:

      Objective To optimize the extraction technology of polysaccharides from Hedysarum semenovii Regelet Herd. Methods Taking the content of polysaccharide as the evaluation index, the extraction time, extraction temperature and the ratio of liquid and material as the investigation factors, the method of combining single factor test and response surface analysis was adopted and then the suitable extraction technology was discussed. Results The optimum extraction conditions of polysaccharides from Hedysarum semenovii Regelet Herd were as follows: 1:32 (m:V), 3 hours and 92 ℃. Under these conditions, the actual verified content of polysaccharide was 6.33%, which was close to the predicted value of 6.39%. Conclusions The selected process is reasonable and feasible, and can be used for the extraction of polysaccharides from Hedysarum semenovii Regelet Herd.