高效液相色谱-四极杆-高分辨飞行时间质谱直接进样测定饮用水中8种微囊藻毒素

Determination of 8 microcystins in drinking water by direct injection using high performance liquid chromatography-quadrupole-high resolution time of flight mass spectrometry

中文关键词:  高分辨飞行时间质谱  微囊藻毒素  直接进样  饮用水

英文关键词:high resolution time of flight mass spectrometry  microcystins  direct injection  drinking water

中文摘要:

      目的 建立了高效液相色谱-四极杆-高分辨飞行时间质谱测定饮用水中8种微囊藻毒素的检测方法。方法 水样经亲水聚四氟乙烯滤膜过滤后, 采用Poroshell 120 EC-C18色谱柱(2.1 mm×100 mm, 2.7 μm)分离, 高效液相色谱-四极杆-高分辨飞行时间质谱检测, 采用高分辨多反应监测扫描模式进行监测, 外标法定量。 结果 8种微囊毒素在0.1~50.0 μg/L线范围内线性关系良好, 相关系数大于0.998以上, 方法的检出限在0.05~0.10 μg/L。在1、5、10 μg/L 3个水平浓度添加时, 其平均回收率在83.2%~105.4%, 相对标准偏差为2.5%~11.8%。结论 该方法简单、快速、灵敏度高、重复性好, 适合于饮用水中微囊藻毒素的日常检测。

英文摘要:

      Objective To establish a method for the determination of 8 microcystins in drinking water by high performance liquid chromatography-quadrupole-high-resolution time-of-flight mass spectrometry. Methods After filtration with hydrophilic polytetrafluoroethylene filter membrane, the water samples were separated by Poroshell 120 EC-C18 chromatographic column (2.1 mm×100 mm, 2.7 μm), detected by high performance liquid chromatograph-four-pole high-resolution time-of-flight mass spectrometry, monitored by high-resolution multiple response monitoring scanning mode, and quantified by external standard method. Results The linear relationships of the 8 microcystis toxins were good in the range of 0.1?50 μg/L, the correlation coefficient was greater than 0.998, and the detection limit of the method was 0.05?0.10 μg/L. The average recoveries at 3 spiked levels (1, 5, 10 μg/L) were 83.2%?105.4%, with the relative standard deviations of 2.5%?11.8%. Conclusions This method is simple, fast, sensitive and reproducible, and is suitable for routine detection of microcystis toxin in drinking water.