基于蛋白组学技术对海参样品的研究

Study on sea cucumber samples based on proteomics

中文关键词:  海参  蛋白组学  提取  酶解  定量分析

英文关键词:sea cucumber  proteomics  extraction  enzymolysis  quantitative analysis

中文摘要:

      随着人们物质生活水平需要的不断提高, 具有高营养价值的海珍品食用安全备受关注。海参是从属于海参纲的棘皮动物, 有研究报道, 海参与人参具有类似的药用特性, 已成为中国、日本等亚洲国家的名贵食材。蛋白质组学(proteomics)是后基因组时代的热点研究领域之一。二维凝胶电泳、高效液相色谱、毛细管电泳及其与质谱联用等已成功应用于蛋白质组分离和鉴定。目前, 采用蛋白质组学方法对海参样品的研究主要是集中在海参育苗、养殖、加工技术对海参组织结构及品质的影响, 以及病害防治方面的基础性研究, 而采用蛋白组学对海参产地、养殖方式差异性研究, 相关报道较少。本文主要从蛋白组学研究海参的现状及海参蛋白的提取、酶解、蛋白的鉴定及定量分析等方面进行了概述, 为海参的育苗育种、繁殖、病害的研究奠定基础。

英文摘要:

      With the continuous improvement of people’s material living standards, the safety of sea treasures with high nutritional value has received much attention. Sea cucumber is an echinoderma belonging to Holothuroidea. It has been reported that sea cucumber and ginseng have similar medicinal properties and has become a valuable ingredient in Asian countries such as China and Japan. Proteomics is one of the hot research areas in the post-genome era. Two-dimensional gel electrophoresis, high performance liquid chromatography, capillary electrophoresis and its combination with mass spectrometry have been successfully applied to proteome separation and identification. At present, the study of sea cucumber samples by proteomics is mainly focused on the effects of sea cucumber seedlings, culture and processing techniques on the structure and quality of sea cucumber, and basic research on disease control. But there were few reports about proteomics used to study the differences of sea cucumber production area and culture mode. In this paper, the current status of sea cucumber and the extraction, enzymatic hydrolysis, protein identification and quantitative analysis of sea cucumber are summarized, which lays a foundation for the research of breeding, propagation and disease of sea cucumber.