利用不确定度比对乳粉中叶酸含量的快速检测方法

Comparison of rapid detection methods of folic acid in milk powder by uncertainty

中文关键词:  乳粉  叶酸  微生物法  酶联免疫法  不确定度

英文关键词:milk powder  folic acid  microbial method  enzyme-linked immunosorbent assay  uncertainty

中文摘要:

      目的 利用不确定度比对乳粉中叶酸含量的快速检测方法。方法 分别用进口和国产的微生物法和酶联免疫法的试剂盒对代表性乳粉样品进行3 d 3平行检测, 对检测数据做正态分布分析及不确定度计算。结果 各方法检测结果正态分布检验值(anderson-darling, AD)均小于临界值0.725(95%置信区间), 服从正态分布; 微生物快速检测方法不确定度范围为13.3%~27.4%; 酶联免疫方法不确定度范围为9.6%~ 31.3%。结论 微生物试剂盒快速检测方法, 具有稳定性较好, 品牌差异较小的特点, 适合质控品的长期监测, 质控图的建立。酶联免疫检测方法, 具有简单、快速的特点, 适合样品中叶酸的快速检测。

英文摘要:

      Objective To compare the rapid detection methods of folic acid in milk powder by uncertainty Methods Representative milk powder samples were tested for 3 d 3 in parallel using imported and domestic microbiological and enzyme-linked immunosorbent assay kits. Normal distribution analysis and uncertainty calculation for test data, and normal distribution analysis and uncertainty calculation were performed. Results The normal distribution test value (anderson-darling, AD) of each method was less than the critical value of 0.725 (95% confidence interval), which indicated the data subjected to normal distribution. The uncertainty of microbial rapid detection method ranged from 13.3% to 27.4%. The uncertainty of the immunization method ranged from 9.6% to 31.3%. Conclusion Rapid detection method of microbial kit has the characteristics of good stability and small brand difference, suitable for long-term monitoring of quality control products and establishment of quality control chart. The enzyme-linked immunosorbent assay has the characteristics of simple and rapid, and is suitable for rapid detection of folic acid in samples.