高效液相色谱法测定保健食品中阿替匹林C的含量

Determination of Artepillin C in health care products by high performance liquid chromatography

中文关键词:  阿替匹林C  高效液相色谱法  保健食品  蜂胶

英文关键词:Artepillin C  high performance liquid chromatography  health care products  propolis

中文摘要:

      目的 建立高效液相色谱法测定保健食品中阿替匹林C的方法。方法 保健食品直接用甲醇超声提取, 加水稀释, 采用Agilent ZORBAX SB-Aq色谱柱分离, 甲醇:1%乙酸水溶液作流动相, 紫外检测器310 nm波长检测, 外标法定量。结果 阿替匹林C在0.80~40.0 μg/mL浓度范围内线性良好, 相关系数为0.9999, 方法回收率为99.2%~102.2%, 相对标准偏差(relative standard deviation, RSD)为1.1%, 检出限(limit of detection, LOD)为0.018 mg/100 g。结论 该方法快速、准确、灵敏, 适合保健食品中阿替匹林C的测定。

英文摘要:

      Objective To establish a method for determining Artepillin C in health food by high performance liquid chromatography Methods Health care products sample were extacted by ultrasonic with methanol and diluted with water, separated by Agilent ZORBAX SB-Aq column, methanol: 1% acetic acid solution as mobile phase, detected by ultraviolet detector at 310 nm wavelength, and quantified by external standard method. Results The calibration curve of Artepillin C showed a good linearity in the range of 0.80?40.0 μg/mL (r=0.9999). The recoveries of Artepillin C were in the range of 99.2%?102.2%. The relative standard deviation was 1.1%, and the limits were 0.018 mg/100 g. Conclusions The method is rapid, accurate and sensitive. It is suitable for the determination of Artepillin C in health care products.