高效液相色谱法测定保健食品中绞股蓝皂苷XLIX的含量

Determination of gypenoside XLIX in health food by high performance liquid chromatography

中文关键词:  高效液相色谱法  绞股蓝皂苷XLIX  保健食品

英文关键词:high performance liquid chromatography  gypenoside XLIX  health food

中文摘要:

      目的 建立高效液相色谱法检测保健食品中绞股蓝皂苷XLIX含量的检测方法。方法 样品经甲醇提取, 采用Ultimate XB-C18柱(4.6 mm×100 mm, 3 μm)进行色谱分离, 以乙腈-水作为流动相进行梯度洗脱, 流速为0.5 mL/min, 检测波长为203 nm, 柱温40 ℃。结果 绞股蓝皂苷XLIX在0.001~1 mg/mL范围内线性关系良好(线性方程: Y=53.06X＋86.3, r=0.999); 绞股蓝皂苷XLIX在0.1、0.2、1.0 g/kg 3个添加水平上进行 加标回收实验, 平均回收率为86.1%~104.9%, 相对标准偏差为1.6%~6.0%, 方法检出限为5 mg/100 g或 5 mg/100 mL, 定量限为10 mg/100 g或10 mg/100 mL。结论 本方法前处理过程简单、分析时间短、准确度高, 适用于保健食品中绞股蓝皂苷XLIX的检测。

英文摘要:

      Objective To establish a method for determination of gypenoside XLIX in health food by high performance liquid chromatography (HPLC). Methods The sample was extracted by methanol and performed on a Ultimate XB-C18 column with gradient elution program of acetonitrile-water. The flow rate was 0.5 mL/min, the detection wavelength was 203 nm, and the column temperature was 40 ℃. Results Gypenoside XLIX had good linear relationships in the range of 0.001?1 mg/mL(Y=53.06X＋86.3, r=0.999). Average recoveries at 3 spiked levels (0.1, 0.2, 1.0 g/kg) were 86.1%?104.9%, and the relative standard deviations (RSDs) were in the ranges of 1.6%?6.0%. The limits of detection (LOD) was 5 mg/100 g or 5 mg/100 mL, and the limits of quantitation (LOQ) was 10 mg/100 g or 10 mg/100 mL. Conclusion This method has the advantages of simple pretreatment, short analysis time and high accuracy, which is suitable for the detection of gypenoside XLIX in health food.