免疫分析多残留同步法测定饲料中硝基呋喃类药物

Determination of nitrofuran drugs in feed by multi-residue immunoassay method

中文关键词:  硝基呋喃  半抗原  多克隆抗体  多残留

英文关键词:nitrofurans  hapten  polyclonal antibody  multiple residues

中文摘要:

      目的 建立酶联免疫吸附多残留同步法测定饲料中硝基呋喃类违禁药物残留的分析方法。方法 采用5-硝基糠醛和对羧基苯肼反应合成含有硝基呋喃类共有结构的半抗原4-硝基呋喃甲醛-(4-羧基-苯基)-腙(4-nitrofuran formaldehyde-(4-carboxyl phenyl) hydrazon, NFHBA)。通过偶联载体蛋白牛血清白蛋白(albumin from bovine serum, BSA)后的免疫原NFHBA-BSA免疫新西兰大白兔, 成功制备了特异性识别呋喃环位点硝基的多克隆抗体。结果 该抗体对4种目前主要使用的硝基呋喃类抗生素药物: 呋喃唑酮、呋喃它酮、呋喃西林、呋喃妥因的检测半抑制浓度(half maximal inhibitory concentration, IC50)分别达5.2、6.4、26.6、4.2 ng/mL, 饲料样品平均添加回收率均在80%~90%之间。结论 该方法能达到产品检测要求, 可用于饲料中硝基呋喃类药物多残留同步快速检测。

英文摘要:

      Objective To establish a method for simultaneous determination of multiple residues of nitrofurans in feed by multi-residue enzyme-linked immunosorbent assay (ELISA) method. Methods The hapten 4-nitrofuran formaldehyde-(4-carboxyl phenyl) hydrazon (NFHBA) with a hydrazone type structure was synthesized by 5-nitro furfural and carboxyl phenylhydrazine. The New Zealand white rabbits were immunized with the immunogen NFHBA-BSA conjugated with the carrier protein bovine serum albumin (BSA), and a polyclonal antibody that specifically recognized the nitro-ring nitro group was successfully achieved. Result The half maximal inhibitory concentration (IC50) values of furazolidone, furaltadone, nitrofurazone, and nitrofurantoin were 5.2, 6.4, 26.6, 4.2 ng/mL, respectively, which were used as main nitrofuran antibiotic drugs. The average recoveries for feed were all 80%?90%. Conclusion This method can meet the requirements of product testing, and can be used for rapid simultaneous detection of multi-residue of nitrofuran drugs in feed.