巧克力中沙门氏菌检验能力验证研究

Research on proficiency testing for the detection of Salmonella in chocolate

中文关键词:  能力验证  沙门氏菌  血清分型

英文关键词:proficiency testing  Salmonella  serotypes

中文摘要:

      目的 对参加中国食品药品检定研究院组织的NIFDC-PT-135巧克力中沙门氏菌检验能力验证进行分析。方法 在参照GB 4789.4-2016《食品国家安全标准 食品微生物学检验 沙门氏菌检验》和能力验证作业指导书传统方法鉴定的基础上, 借助miniVIDAS和VITEK2 2种仪器进行检验分析。结果 CODE0002未检出沙门氏菌、CODE0485检出肠沙门菌双相亚利桑那亚种, CODE0633检出鼠伤寒沙门氏菌。结论 本次能力验证实验结果为满意。实验室在进行沙门氏菌检验时可以使用仪器与传统分析方法相结合的检验方法, 但是要注意一些细节问题, 防止漏检和误检。

英文摘要:

      Objective To analyze the verification of inspection capability of Salmonella in NIFDC-PT-135 chocolate organized by National Institutes for Food and Drug Control. Methods On the basis of referring to GB 4789.4-2016 National food safety standard food microbiology test salmonella test and the traditional method identification of ability verification operating instructions, isolation and identification of Salmonella were conducted by using miniVIDAS and VITEK2. Results Salmonella was not detected in CODE0002, Salmonella enterum biphasic Arizona subspecies was detected in CODE0485, and Salmonella typhimurium was detected in CODE0633. Conclusion The results of this capability verification experiment are satisfactory. The laboratory can use the test method combined with the traditional analysis method when conducting the Salmonella test, but there are some details that should be taken into account to prevent missed or false checks.