蛋白芯片技术在食品安全检测中的应用进展

Progress in the applications of protein chip in food safety detection

中文关键词:  蛋白芯片  食品安全检测  综述

英文关键词:protein chip  food safety detection  review

中文摘要:

      随着生命科学与生物技术的快速发展, 国际上农畜产品安全检测技术正在向着检测方法与技术的通量化、快速化、自动化和智能化等方向发展。而免疫检测方法由于其具有特异性和灵敏性、其试剂具有稳定性、操作具有简便性, 因此适合推广应用。其中蛋白芯片法与传统酶联免疫吸附(enzyme-linked immuno sorbent assay, ELISA)方法比较, 具有高通量, 样品用量少, 一份样品可同时进行多指标分析, 大大降低了检测的成本, 提高了检测的效率, 并且检测限与灵敏度等均与ELISA方法相当。动物源性食品种类和成分较为复杂, 目标化合物的检测限较低, 各目标化合物的性质差异大, 且可能同时存在多种组分。因此, 近年来蛋白芯片在食品安全检测等领域的应用也日渐增多。本文就蛋白芯片技术在食品安全检测中的应用进行综述, 以期为食品安全现场快速检测提供参考。

英文摘要:

      With the rapid development of life sciences and biotechnology, the international safety testing technology for agricultural and livestock products is developing towards the high throughput, rapid, automated and intelligent of the detection methods and technologies. The immunoassay method is suitable for popularization and application because of its specificity and sensitivity, its reagent stability and ease of operation. Compared with the traditional ELISA method, the protein chip method has high throughput and small sample consumption, and one sample can simultaneously perform multi-index analysis, which greatly reduces the cost of detection, improves the detection efficiency, and has the limit of detection and sensitivity equivalent to the ELISA method. The types and components of animal-derived foods are complex, the limit of detection of target compounds is low, the properties of each target compound vary greatly, and multiple components may exist at the same time. Therefore, in recent years, the application of protein chips in food safety testing and other fields has also increased. This article reviewed the application of protein chip technology in food safety detection, in order to provide a reference for food safety field rapid detection.