诃子膏核心药对正常大鼠能量代谢相关因子的影响

Effects of Triphala core drugs on energy metabolism related factors in normal rats

中文关键词:  维药  诃子膏  诃子  毛诃子  余甘子  能量代谢

英文关键词:Uyghur medicine  Triphala  Terminalia chebula Retz.  Terminaliae belliricae fructus  Phyllanthus emblica Linn.  energy metabolism

中文摘要:

      目的 分别观察诃子膏3种核心药(诃子、毛诃子、余甘子)及其组合(剂量比为1:1:1)对正常大鼠能量代谢相关因子的影响, 分析其作用趋势和特点。方法 制备药液并灌胃给药30 d后, 观察大鼠体重变化, 采用酶联免疫吸附法(enzyme-linked immunosorbent assay, ELISA)检测肝组织Na+-K+ ATP酶、Ca2+-Mg2+ ATP酶和琥珀酸脱氢酶(succinate dehydrogenase, SDH)活性、肝糖原(hepatic glycogen, HG)和肌糖原(muscle glycogen, MG)含量, 检测血浆三碘甲状腺原氨酸(triiodothyronine, T3)、四碘甲状腺原氨酸(tetraiodothyronine, T4)、促甲状腺激素(thyroid stimulating hormone, TSH)含量。结果 核心药组和毛诃子组能够抑制正常老鼠体重自然增大。毛诃子组显著升高Na+-K+ ATP酶、Ca2+-Mg2+ ATP酶和SDH活性, HG、MG、T3、T4和TSH含量, 而诃子组、余甘子组和核心药组合对能量代谢相关因子的影响不明显。结论 核心药组合对正常大鼠Na+-K+ ATP酶、Ca2+-Mg2+ ATP酶和SDH、HG、MG、T3、T4和TSH等能量代谢相关因子的影响不显著, 而毛诃子影响显著, 可能是毛诃子所含物质基础与其他2种药材不同有关, 还需要进一步研究。

英文摘要:

      Objective To observe the effects of 3 core drugs (Terminalia chebula Retz., Terminaliae belliricae fructus, and Phyllanthus emblica Linn.) and their combinations of Triphala compound (dose ratio 1:1:1) on energy metabolism related factors in normal rats, and analyze their action trends and characteristics. Methods After the preparation of the drug solution and intragastric administration for 30 d, the body weight changes of rats were observed. The content of Na+-K+ ATPase, Ca2+-Mg2+ ATPase, succinate dehydrogenaseactivity (SDH), hepatic glycogen (HG), muscle glycogen (MG) in liver tissue and the plasma triiodothyronine (T3), tetraiodothyronine (T4), thyroid stimulating hormone (TSH) were detected by enzyme-linked immunosorbent assay (ELISA). Results The core drug combination group and the Terminaliae belliricae fructus group inhibited the natural weight gain of the normal rats after administration, and the levels of Na+-K+ ATPase, Ca2+-Mg2+ ATPase and SDH, HG, MG, T3, T4 and TSH were significantly increased in the Terminaliae belliricae fructus group, while the effects of core drug combinations group on energy metabolism related factors were not obvious. Conclusions The core drug combination has no significant effect on Na+-K+ ATPase, Ca2+-Mg2+ ATPase, SDH, HG, MG, T3, T4, TSH and other energy metabolism related factors in normal rats, while the effect of Terminaliae belliricae fructus is significant, suggesting that the material basis of Terminaliae belliricae fructus might be related to the difference between the other 2 drugs, which needs further study.