可视化膜芯片检测转基因大豆的研究

Detection of transgenic soybean by visible membrane chip

中文关键词:  转基因大豆  可视化  膜芯片  检测

英文关键词:transgenic soybean  visible  membrane chip  detection

中文摘要:

      目的 研究可视化膜芯片技术对转基因大豆及其制品的检测能力, 验证该技术的灵敏度及可行性。方法利用可视化膜芯片对转基因大豆及其制品进行检测, 并采用行业标准要求的实时荧光PCR方法进行对比验证。结果 该技术具备典型外源基因片段的判定能力, 能够用于大豆及其制品中转基因成分的检测, 方法检出限为0.1%, 比对验证实际样品的检测结果与行业标准荧光PCR法一致。结论 该技术便捷、直观、准确, 可用于大豆及其制品的转基因成分快速初筛与鉴定。

英文摘要:

      Objective To investigate the detection ability of visible membrane chip technology for transgenic soybean and its products, and to verify the sensitivity and feasibility of this technology. Methods The transgenic soybean and its products were detected by visual membrane chip, and the real-time fluorescent PCR method required by industry standard was used to verify the results. Results The technology had the ability to identify typical exogenous gene fragments and could be used to detect transgenic components in soybean and the products. The limit of detection for this method was 0.1%. The results of comparison and verification of actual samples were consistent with those of industry standard fluorescent PCR. Conclusion This technology is convenient, intuitive and accurate, which can be used for rapid screening and identification of genetically modified ingredients in soybean and the products.