动物源性食品中磺胺类药物残留能力验证关键技术点分析

Analysis of proficiency testing for sulfonamides residues in animal-derived food

中文关键词:  能力验证  动物源性食品  兽药残留  液相色谱-串联质谱法

英文关键词:proficiency testing  animal-derived food  drug residues  liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 通过参加能力验证, 提高实验室对动物源性食品中磺胺类药物残留能力验证的检测能力。方法 依据能力验证作业指导书并参照GB/T 20759-2006及农业部1077号公告-1-2008针对不同样品基质, 设计合适的检测实验方案, 对样品进行前处理, 采用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry, LC-MS/MS)进行检测。结果 样品S18-10、H-18-05检验结果与能力验证组织单位结果一致, 能力验证过程中的关键点如内标的添加、样品均质、质控样的使用及质谱检测定性定量的准确性等尤为重要。结论 通过能力验证可以确保日常检测结果准确可靠, 同时还可以提高实验室检测人员的技术水平, 加强实验室质量控制。

英文摘要:

      Objective To effectively improve the capability of detection techniques for sulfonamides residues in animal-derived food by participating in capacity verification. Methods Appropriate test protocols were designed for different sample substrates according to the capability verification work instruction and in accordance with GB/T 20759-2006 and Ministry of Agriculture announcement No. 1077-1-2008. After pretreatment, samples were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results The results of S18-10 and H-18-05 were consistent with the results of the organization unit for capacity verification. Key points in the process of capability verification, such as addition of internal standard, sample homogeneity, use of quality control sample and qualitative and quantitative accuracy of mass spectrometry detection were particularly important. Conclusion The ability verification can ensure the accuracy and reliability of daily test results, improve the technical level of laboratory testing personnel, and strengthen the quality control of laboratory