农作物中抗生素检测技术的研究进展

Research progress in determination methods of antibioticsresidues in plants

中文关键词:  抗生素  农作物  检测技术

英文关键词:antibiotics  crops  detection technology

中文摘要:

      随着养殖业的规模化和集约化发展, 抗生素作为疾病预防与治疗药物、生长促进剂、饲料添加剂等被广泛用于畜禽养殖和水产养殖业中, 进而进入水环境和土壤环境, 被农作物根系吸收进入茎、叶中并在植物体内富集, 从而通过食物链进入人体内。近年来研究者对农作物中抗生素的研究逐渐增多。目前对农作物中兽用抗生素残留的前处理技术主要包括QuEChERs方法和固相萃取法, 检测技术主要包括高效液相色谱法、高效液相色谱-串联质谱法和酶联免疫法。高效液相色谱-串联质谱法具有高灵敏度、高选择性和高准确度, 可同时快速测定多个目标物, 更适合农作物中兽用抗生素残留的检测。本文对环境中抗生素的来源、农作物中抗生素污染的现状、抗生素残留检测的提取、净化方法以及检测方法的研究进展进行了综述。

英文摘要:

      With the large-scale and intensive development of livestock breeding, antibiotics as disease prevention and treatment drugs, growth stimulant and feed additive, etc, have been widely used in livestock breeding and aquaculture, and then permeated through the water and soil environment, absorbed by roots of crops into stems and leaves and enriched in crops, thereby antibiotics enter the human body through the food chain. In recent years, research on veterinary antibiotics in crop has gradually increased. At present, the pretreatment techniques for veterinary antibiotic residues in crops mainly include QuEChERs method and solid phase extraction method. The detection technologies mainly include high performance liquid chromatography, high performance liquid chromatography-tandem mass spectrometry and enzyme-linked immunosorbent assay. High performance liquid chromatography-tandem mass spectrometry with high sensitivity, high selectivity and high accuracy, can simultaneously measure multiple targets and is more suitable for detection of veterinary antibiotic residues in crops. The paper reviewed the source of antibiotics in the environment, the status of antibiotic pollution in crops, and the research progress of the extraction, purification and detection methods of antibiotics residues in plants.