离子色谱法测定香菇中葡萄糖含量的不确定度评定

Uncertainty evaluation of determination of glucose in mushroom by ion chromatography

中文关键词:  不确定度  离子色谱法  香菇  葡萄糖

英文关键词:uncertainty  ion chromatography  mushroom  glucose

中文摘要:

      目的 评定离子色谱法测定香菇中葡萄糖含量的不确定度。方法 依据JJF 10591.1-2012《测量不确定度评定与表示》, 考察称量、标准溶液配制、标准曲线拟合、回收率等主要因素引入的不确定度, 并对不确定度的各分量进行计算和合成。结论 当香菇中葡萄糖添加量为3.29 mg/kg时, 在95%的置信区间下, 其扩展不确定度为0.30 mg/kg (k=2)。评定结果表明, 实验过程的不确定度主要来源于校准曲线以及回收率。结论 该方法准确可靠, 适用于离子色谱法测定香菇中葡萄糖含量的不确定度评定, 对检测结果准确度的提高具有指导意义。

英文摘要:

      Objective To evaluate the uncertainty of determination of glucose in mushroom by ion chromatography. Methods According to JJF 1059.1-2012 Measurement uncertainty evaluation and presentation, the uncertainty of main factors such as weighing, standard solution preparation, standard curve fitting and recovery were investigated, and the components of uncertainty were calculated and synthesized. Results When the content of glucose in mushroom was 3.29 μg/kg, the extended uncertainty was 0.30 μg/kg (k=2) under the 95% confidence interval. The evaluation results showed that the uncertainties of the experiment were mainly derived from curves calibration and recovery rates. Conclusion The evaluation method is accurate and reliable, which is suitable for the evaluation of uncertainty of determination of glucose in mushroom by ion chromatography. It has guiding significance to improve the accuracy of test results.