益气复生方对脂多糖诱导胃癌细胞SGC-7901炎症的改善作用

Effect of Yiqi Fusheng Decoction on the improvement of SGC-7901SGC-7901 inflammation induced by LPS in gastric cancer cells

中文关键词:  益气复生方  胃癌细胞  炎症因子

英文关键词:Yiqi Fusheng Decoction  gastric cancer cell  inflammatory factor

中文摘要:

      目的 考察益气复生方对脂多糖(lipopolysaccharide, LPS)诱导的胃癌细胞SGC-7901SGC-7901增值作用的影响及对其产生的炎症因子的改善作用。方法 培养人胃癌细胞SGC-7901, 用LPS诱导SGC-7901细胞, 用MTS法检测不同浓度的LPS对SGC-7901细胞活力的影响, 用浓度为250、50、10 mg/mL益气复生方作用于细胞, 采用ELISA法检测其细胞上清液中炎症因子的释放量。结果 益气复生方能够显著降低SGC-7901SGC-7901细胞的存活率, 对胃癌细胞起到很好的抑制作用, 能够显著改善LPS诱导的胃癌细胞肿瘤坏死因子(TNF-α)、白介素6(IL-6)、白介素8(IL-8)炎症因子的释放, 能够促进干扰素(IFN-γ)的释放量。 结论 益气复生方可以抑制LPS刺激的SGC-7901SGC-7901细胞炎症因子的释放, 对胃癌细胞具有一定的改善作用。

英文摘要:

      Objective To investigate the effect of Yiqi Fusheng Decoction on the proliferation of LPS induced gastric cancer cell line SGC-7901SGC-7901 and the improvement of its inflammatory factors. Methods Human gastric cancer cell SGC-7901 was cultured, and SGC-7901 cells were induced by LPS. The effect of different concentrations of LPS on the viability of SGC-7901 cells was detected by MTS. The cells were treated with 250, 50, and 10 mg/mL of Yiqi Fusheng Decoction, and the release of inflammatory cytokines in the supernatant was detected by ELISA. Results Yiqi Fusheng Decoction could significantly reduce the survival rate of SGC-7901SGC-7901 cells and played a good role in inhibiting gastric cancer cells. It could significantly improve the release of inflammatory factors of tumor necrosis factor (TNF-α), interleukin 6 (IL-6) and IL-8 induced by LPS in gastric cancer cells, and could promote the release of interferon (IFN-γ). Conclusion Yiqi Fusheng Decoction can inhibit the release of inflammatory factors of SGC-7901 cells stimulated by LPS, and can improve the gastric cancer cells.