流式细胞仪快速检测酸奶中乳酸菌含量

Determination the content of lactic acid bacteria in yoghurt by flow cytometry

中文关键词:  酸奶  流式细胞仪  乳酸菌  快速检测

英文关键词:yogurt  flow cytometry  lactic acid bacteria  rapid detection

中文摘要:

      目的 建立一种使用流式细胞仪检测酸奶中乳酸菌含量的快速检测方法。方法 吸取稀释后样品匀液100 μL加入20 mL试管中, 30 min内使用流式细胞仪检测。经过光电倍增器过滤和检测, 荧光脉冲的密度和高度被记录下来并被换算为细菌的个数。结果 与国标法相比, 仪器检测时间约缩短了3 d, 试验结果表明流式细胞仪检测结果与国标结果R值均在1以下。结论 流流式细胞仪法与国标平皿菌落计数法的结果具有很高的一致性。采用流式细胞仪对酸奶中的乳酸菌进行快速检测, 培养时间为由48 h缩短至13 min, 大大提高了检测速度, 对于乳制品生产企业来说, 意义重大。

英文摘要:

      Objective To establish a rapid method for the determination of lactic acid bacteria in yoghurt by flow cytometry. Methods The diluted sample of 100 μL was absorbed into 20 mL test tube, and it was test on the flow cytometry within 30 min. The density and height of the fluorescent pulse were recorded and converted to the number of bacteria after the photomultiplier was filtered and detected. Results Compared with the national standard method, the detection time of the instrument was shortened by 3 d, and the results of the flow cytometry and the national standard showed that the R values were all below 1. Conclusion The results of flow cytometry are consistent with those of the national standard colony count method. Using flow cytometry to detect the lactic acid bacteria in yogurt, can shorten the culture time from 48 h to 13 min, which greatly improves the detection speed. It is of great significance to dairy enterprises.