全自动氨基酸分析仪测定支链氨基酸粉中牛磺酸的含量

Determination of taurine in branched amino acid powder by automatic amino acid analyzer

中文关键词:  牛磺酸  支链氨基酸粉  保健食品  氨基酸分析仪

英文关键词:taurine  branched chain amino acid powder  health food  amino acid analyzer

中文摘要:

      目的 建立全自动氨基酸分析仪测定支链氨基酸粉中牛磺酸。方法 样品经上样缓冲液(pH 2.2)溶解提取, 按照氨基酸分析仪设定的钠体系程序洗脱, 用Na型离子交换色谱柱分离, 与茚三酮反应液在135 ℃下反应, 生成可在570 nm和440 nm检测到的蓝色物质, 采用外标法定量。结果 牛磺酸在4.1065~20.5326 μg/mL浓度范围内有良好的线性, r2=0.999, 回收率为101.28%, RSD为2.1%, 检出限为15 mg/100 g。结论 该方法准确可靠, 不需要剧毒衍生试剂, 适用于支链氨基酸粉中牛磺酸的测定。

英文摘要:

      Objective To establish a method for determination of taurine in branched amino acid powder by automatic amino acid analyzer. Methods The sample was extracted by loading buffer (pH 2.2), and eluted according to the sodium system procedure by the amino acid analyzer, separated by Na-ion exchange chromatographic column, and reacted with ninhydrin at 135 ℃. The reaction was carried out at 570 nm and 440 nm of the blue material, using external standard method for quantitation. Results Taurine had a good linear relationship in the range of 4.1065~20.5326 μg/mL (r2=0.9999), the average recovery was 101.28%, RSD was 2.1%, and the limit of detection was 15 mg/100 g. Conclusion The method is accurate and reliable, and do not need toxic reagents, which is suitable for the determination of taurine in branched chain amino acid powder.