酶联免疫法检测酱油中的黄曲霉毒素B1

Detection of aflatoxin B1 in soy sauce by enzyme linked immunosorbent assay

中文关键词:  酶联免疫法  酱油  黄曲霉毒素B1

英文关键词:enzyme linked immunosorbent assay  soy sauce  aflatoxin B1

中文摘要:

      目的 建立酶联免疫法检测酱油中黄曲霉毒素B1(Aflatoxin B1, AFB1)的分析方法。方法 酱油样本经纯乙腈(料液比=1:2, V:V)提取, 再做1:9稀释, 通过酶联免疫吸附法(enzyme linked immunosorbent assay, ELISA)测定样本中AFB1。结果 当加标浓度为2 μg/kg和5 μg/kg时, 纯乙腈对酱油中AFB1的提取回收率结果分别为121.3%和106.5%; 方法检出限为1 μg/kg。与国标方法检测结果的相对标准偏差小于10%。结论 本方法准确、灵敏度高, 可适用于酱油中AFB1的检测。

英文摘要:

      Objective To establish a method for the detection of aflatoxin B1 in soy sauce by enzyme linked immunosorbent assay (ELISA). Methods Soy sauce was extracted with acetonitrile (1:2, V:V), and processed with 1:9 dilution, and then the content of AFB1 in samples was determined by ELISA. Results When the spiked concentrations were 2 μg/kg and 5 μg/kg, the recoveries of AFB1 in soy sauce extracted by pure acetonitrile were 121.3% and 106.5%, respectively. The limit of detection was 1 μg/kg. Compared with the national standard method, the relative standard deviation of result was less than 10%. Conclusion This method is accurate and sensitive, which is suitable for the detection of AFB1 in soy sauce.