国标培养法与2种金黄色葡萄球菌快速筛检方法的比较

Comparison of traditional culture method and 2 rapid screening methods for detection of Staphylococcus aureus

中文关键词:  金黄色葡萄球菌  mini-VIDAS法  膜芯片法  快速筛检

英文关键词:Staphylococcus aureus  mini-VIDAS method  membrane chip method  rapid screening

中文摘要:

      目的 将mini-VIDAS法和膜芯片2种快速筛检方法与国标培养法进行比较, 评估2种快速筛检方法的效果和可操作性。方法 以金黄色葡萄球菌为目标菌, 针对3种筛检方法设计灵敏性、特异性、抗干扰性实验和人工污染实验, 通过比较获得3种筛检方法的应用效果情况。结果 国标培养法和mini-VIDAS法的灵敏性较好, mini-VIDAS法和膜芯片法的特异性和抗干扰性较好, 人工污染实验中3种方法的结果均符合设计预期。结论 日常检验中可采用国标培养法和mini-VIDAS法进行筛检工作, 应急任务可使用膜芯片法, 以缩短筛检时间, mini-VIDAS法和膜芯片法是金黄色葡萄球菌快速筛检的可靠工具。

英文摘要:

      Objective To compare 2 rapid screening methods of mini-VIDAS method and membrane chip method with the culture method in national standard, evaluating the effectiveness and feasibility of the 2 methods. Methods Staphylococcus aureus was selected as target bacteria, the experiments were designed to test the sensitivity, specificity and interference resistance of 3 methods, and the artificially contaminated samples were tested simultaneously. The application effects of 3 screening methods were compared. Results The culture method in national standard and mini-VIDAS method had a high sensitivity, the specificity and interference resistance of mini-VIDAS and membrane chip method were better, and the results of 3 methods in artificially contaminate test were consistent with the design expectation. Conclusion The culture method in national standard and mini-VIDAS method can be used in daily inspections, and membrane chip method can be used in emergency testing in order to shorten the screening time. Additionally, the mini-VIDAS method and membrane chip method are reliable tools for rapid screening of Staphylococcus aureus.