薄层色谱法和气相色谱法分析2种侧耳多糖的单糖组成

Analysis of monosaccharide compositions of 2 kinds of Pleurotus polysaccharides by thin layer chromatography and gas chromatography

中文关键词:  阿魏菇  杏鲍菇  多糖  单糖  薄层色谱法  气相色谱法

英文关键词:Pleurotus ferulae Lanzi  Pleurotus eryngii Quél  polysaccharide  monosaccharide  thin layer chromatography  gas chromatography

中文摘要:

      目的 分析阿魏菇和杏鲍菇2种侧耳多糖的单糖组成。方法 阿魏菇多糖和杏鲍菇多糖经完全酸水解后, 用薄层色谱法(thin layer chromatography, TLC)和气相色谱法(gas chromatography, GC)分析样品的单糖组成, 并测定各单糖的摩尔比例。结果 TLC法和GC法均检测出阿魏菇多糖和杏鲍菇多糖含有阿拉伯糖(Ara)、甘露糖(Man)、葡萄糖(Glc)和半乳糖(Gal)单糖组分, GC法测得阿魏菇多糖和杏鲍菇多糖中各单糖的摩尔比(Ara:Man:Glc:Gal)分别为1.00:1.75:11.79:2.22和1.00:4.53:1.92:2.24。结论 阿魏菇多糖和杏鲍菇多糖的单糖组成类似, 但二者的各单糖摩尔比例有差异, TLC和GC法适用于分析阿魏菇和杏鲍菇多糖的单糖组成。

英文摘要:

      Objective To analyze the monosaccharide compositions of polysaccharides from Pleurotus ferulae Lanzi and Pleurotus eryngii Quél. Methods After completely hydrolyzed by trifluoroacetic acid (TFA), the monosaccharide compositions of Pleurotus ferulae Lanzi polysaccharide and Pleurotus eryngii Quél. polysaccharide were analyzed by thin layer chromatography (TLC) and gas chromatography (GC), and the molar ratios of monosaccharides were determined. Results The monosaccharide compositions of 2 kinds of polysaccharides were D-arabinose, D-mannose, D-glucose and D-galactose determine by TLC and GC. The molar ratios of monosaccharides (Ara:Man:Glc:Gal) in 2 kinds of polysaccharides were respectively 1.00:1.75:11.79:2.22 and 1.00:4.53:1.92:2.24. Conclusion The monosaccharide compositions of Pleurotus ferulae Lanzi polysaccharide and Pleurotus eryngii Quél. polysaccharide were similar, however, there was a obvious difference between their molar ratios of monosaccharides. The TLC and GC methods can be used for the monosaccharide compositions analysis of Pleurotus ferulae Lanzi polysaccharide and Pleurotus eryngii Quél. polysaccharide.