耶拿荧光定量PCR在转基因饲料检测中的应用

Application of Jena fluorescence quantitative PCR in the detection of genetically modified feed

中文关键词:  荧光定量PCR  转基因  饲料

英文关键词:fluorescence quantitative PCR  transgenosis  feed

中文摘要:

      目的 优化耶拿荧光定量PCR在转基因饲料检测中的检测条件。方法 采用已知转基因品系的玉米蛋白粉样品, 通过选择常用的不同品牌的Premix溶液、8连管及不同的反应程序进行Ct值对比, 得出最佳的检测方案。结果 最佳的检测方案如下: Takara Premix溶液、LightCycler 8-Tube Strips (white) 八连管及95 ℃ 30 s, 95 ℃ 5 s-60 ℃ 30 s, 40个循环的反应程序, 在此条件下可检测出饲料产品中痕量的转基因成分。结论 耶拿荧光定量PCR对饲料中痕量转基因成分的检测结果可靠, 适用于饲料转基因成分的检测。

英文摘要:

      Objective To optimize the detection conditions of Jena fluorescent quantitative PCR for detection of genetically modified feed. Method Using the corn gluten meal samples with known genetically modified strains, 2 brands of Premix solutions, 8-Tube Strip and different reaction procedures were selected to perform Ct value comparison to obtain the best detection scheme. Results The optimum detection scheme was as follows: Takara Premix solution, LightCycler 8-Tube Strips (white) and 95 ℃ 30 s, 95 ℃ 5 s~60 ℃ 30 s for 40 cycles, and trace amounts of genetically modified components in feed products could be detected. Conclusion The detection result of the genetically modified components in feed detected by Jena fluorescent quantitative PCR is reliable, which is suitable for the detection of genetically modified components in feed.